Metabolic editing: small measures, great impact.

CRISPR-Cas Systems Gene Editing Metabolic Engineering Protein Processing, Post-Translational

Journal

Current opinion in biotechnology
ISSN: 1879-0429
Titre abrégé: Curr Opin Biotechnol
Pays: England
ID NLM: 9100492

Informations de publication

Date de publication:
10 2019
Historique:
received: 26 09 2018
revised: 25 01 2019
accepted: 04 02 2019
pubmed: 9 3 2019
medline: 11 2 2020
entrez: 9 3 2019
Statut: ppublish

Résumé

Metabolic pathways are tightly regulated at the transcriptional and post-translational level, often relying on protein-protein interactions or post-translational protein modifications. Whereas these principles have been established already for a long time, the number of experimentally established cases is expected to rise exponentially in the near future as a result of recent advances in protein-based detection methods. Interactions and modifications are often dependent on only short amino-acid sequences that represent excellent targets for new gene editing technologies by which specific base pairs can be exchanged. Here, we introduce the concept of metabolic editing, which is based on identifying specific amino-acid sequences that are subsequently targeted for gene editing. The proposed workflow will serve for both applied metabolic engineering purposes and proof-of-concept studies in fundamental research.

Identifiants

DOI: 10.1016/j.copbio.2019.02.002 PMID: 30849665
pubmed: 30849665
pii: S0958-1669(18)30151-4
doi: 10.1016/j.copbio.2019.02.002
pii:
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't Review

Langues

eng

Sous-ensembles de citation

IM

Pagination

16-23

Informations de copyright

Copyright © 2019 Elsevier Ltd. All rights reserved.

Auteurs

Gwen Swinnen (G)

Ghent University, Department of Plant Biotechnology and Bioinformatics, Technologiepark 71, B-9052 Ghent, Belgium; VIB-UGent Center for Plant Systems Biology, Technologiepark 927, B-9052 Ghent, Belgium.

Alain Goossens (A)

Ghent University, Department of Plant Biotechnology and Bioinformatics, Technologiepark 71, B-9052 Ghent, Belgium; VIB-UGent Center for Plant Systems Biology, Technologiepark 927, B-9052 Ghent, Belgium. Electronic address: algoo@psb.ugent.be.

Maite Colinas (M)

Ghent University, Department of Plant Biotechnology and Bioinformatics, Technologiepark 71, B-9052 Ghent, Belgium; VIB-UGent Center for Plant Systems Biology, Technologiepark 927, B-9052 Ghent, Belgium. Electronic address: macol@psb.ugent.be.

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Classifications MeSH

questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Épigenèse génétique › Extinction de l'expression des gènes › Systèmes CRISPR-Cas › Metabolic editing: small measures, great impact.
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Génie génétique › Édition de gène › Metabolic editing: small measures, great impact.
questionsmedicales.fr › Technologie, industrie et agriculture › Ingénierie › Bioingénierie › Ingénierie cellulaire › Génie métabolique › Metabolic editing: small measures, great impact.
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Modification traductionnelle des protéines › Maturation post-traductionnelle des protéines › Metabolic editing: small measures, great impact.