The fungal ribonuclease-like effector protein CSEP0064/BEC1054 represses plant immunity and interferes with degradation of host ribosomal RNA.
Amino Acid Sequence
Ascomycota
/ pathogenicity
Fungal Proteins
/ chemistry
Gene Expression Regulation, Fungal
Host-Pathogen Interactions
/ immunology
Plant Diseases
/ immunology
Plant Immunity
/ immunology
Plants
/ immunology
Protein Conformation
RNA, Plant
/ genetics
RNA, Ribosomal
/ genetics
Sequence Homology
Journal
PLoS pathogens
ISSN: 1553-7374
Titre abrégé: PLoS Pathog
Pays: United States
ID NLM: 101238921
Informations de publication
Date de publication:
03 2019
03 2019
Historique:
received:
10
05
2018
accepted:
06
02
2019
revised:
15
04
2019
pubmed:
12
3
2019
medline:
11
5
2019
entrez:
12
3
2019
Statut:
epublish
Résumé
The biotrophic fungal pathogen Blumeria graminis causes the powdery mildew disease of cereals and grasses. We present the first crystal structure of a B. graminis effector of pathogenicity (CSEP0064/BEC1054), demonstrating it has a ribonuclease (RNase)-like fold. This effector is part of a group of RNase-like proteins (termed RALPHs) which comprise the largest set of secreted effector candidates within the B. graminis genomes. Their exceptional abundance suggests they play crucial functions during pathogenesis. We show that transgenic expression of RALPH CSEP0064/BEC1054 increases susceptibility to infection in both monocotyledonous and dicotyledonous plants. CSEP0064/BEC1054 interacts in planta with the pathogenesis-related protein PR10. The effector protein associates with total RNA and weakly with DNA. Methyl jasmonate (MeJA) levels modulate susceptibility to aniline-induced host RNA fragmentation. In planta expression of CSEP0064/BEC1054 reduces the formation of this RNA fragment. We propose CSEP0064/BEC1054 is a pseudoenzyme that binds to host ribosomes, thereby inhibiting the action of plant ribosome-inactivating proteins (RIPs) that would otherwise lead to host cell death, an unviable interaction and demise of the fungus.
Identifiants
pubmed: 30856238
doi: 10.1371/journal.ppat.1007620
pii: PPATHOGENS-D-18-00963
pmc: PMC6464244
doi:
Substances chimiques
Fungal Proteins
0
RNA, Plant
0
RNA, Ribosomal
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e1007620Subventions
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/K021435/1
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/H001646/1
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/M000710/1
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/C513218/1
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/E000983/1
Pays : United Kingdom
Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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