Assays for the Enzymes Catalyzing the O-Acetylation of Bacterial Cell Wall Polysaccharides.
Acetylation
Acetylesterase
/ metabolism
Acetyltransferases
/ metabolism
Bacteria
/ chemistry
Bacterial Proteins
/ metabolism
Cell Wall
/ chemistry
Chromatography, High Pressure Liquid
/ methods
Enzyme Assays
/ methods
Mass Spectrometry
/ methods
Peptidoglycan
/ chemistry
Polysaccharides, Bacterial
/ chemistry
Chitooligosaccharides
O-Acetylation
O-acetylesterase
O-acetyltransferase
Peptidoglycan
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2019
2019
Historique:
entrez:
14
3
2019
pubmed:
14
3
2019
medline:
23
7
2019
Statut:
ppublish
Résumé
The polysaccharides that comprise bacterial cell walls are commonly O-acetylated. This modification confers resistance to hydrolases of innate immune systems and/or controls endogenous autolytic activity. Herein, we present protocols for the compositional analysis of bacterial cell wall O-acetylation, and assays for monitoring O-acetyltransferases and O-acetylesterases. The assays are amenable for the development of high-throughput screens in search of inhibitors of the respective enzymes.
Identifiants
pubmed: 30864128
doi: 10.1007/978-1-4939-9154-9_10
doi:
Substances chimiques
Bacterial Proteins
0
Peptidoglycan
0
Polysaccharides, Bacterial
0
Acetyltransferases
EC 2.3.1.-
Acetylesterase
EC 3.1.1.6
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
115-136Subventions
Organisme : CIHR
Pays : Canada