The Nucleotide-Dependent Interactome of Rice Heterotrimeric G-Protein α -Subunit.
heterotrimeric G protein
rice
tandem mass spectrometry
Journal
Proteomics
ISSN: 1615-9861
Titre abrégé: Proteomics
Pays: Germany
ID NLM: 101092707
Informations de publication
Date de publication:
05 2019
05 2019
Historique:
received:
05
11
2018
revised:
12
02
2019
pubmed:
14
3
2019
medline:
30
4
2020
entrez:
14
3
2019
Statut:
ppublish
Résumé
The rice heterotrimeric G-protein complex, a guanine-nucleotide-dependent on-off switch, mediates vital cellular processes and responses to biotic and abiotic stress. Exchange of bound GDP (resting state) for GTP (active state) is spontaneous in plants including rice and thus there is no need for promoting guanine nucleotide exchange in vivo as a mechanism for regulating the active state of signaling as it is well known for animal G signaling. As such, a master regulator controlling the G-protein activation state is unknown in plants. Therefore, an ab initio approach is taken to discover candidate regulators. The rice Gα subunit (RGA1) is used as bait to screen for nucleotide-dependent protein partners. A total of 264 proteins are identified by tandem mass spectrometry of which 32 were specific to the GDP-bound inactive state and 22 specific to the transition state. Approximately, 10% are validated as previously identified G-protein interactors.
Identifiants
pubmed: 30866160
doi: 10.1002/pmic.201800385
doi:
Substances chimiques
Nucleotides
0
Protein Subunits
0
Guanosine Diphosphate
146-91-8
Guanosine Triphosphate
86-01-1
Heterotrimeric GTP-Binding Proteins
EC 3.6.5.1
Types de publication
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
e1800385Informations de copyright
© 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.