Transcriptome Studies in Trypanosoma cruzi Using RNA-seq.
Bioinformatic pipeline
Differential expression
Gene expression
RNA-seq
Trypanosoma cruzi
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2019
2019
Historique:
entrez:
15
3
2019
pubmed:
15
3
2019
medline:
25
7
2019
Statut:
ppublish
Résumé
RNA-seq is a powerful method to study in detail transcriptome changes in defined conditions, providing enormous amount of information on RNA stability and gene regulation. In this chapter, we describe a directional and a nondirectional library preparation protocol for RNA-seq in Trypanosoma cruzi, as well as a pipeline for bioinformatic analysis, which includes read trimming, alignment to a reference genome, and differential expression.
Identifiants
pubmed: 30868517
doi: 10.1007/978-1-4939-9148-8_3
doi:
Substances chimiques
RNA, Protozoan
0
Types de publication
Journal Article
Langues
eng