Extraction of Highly Degraded DNA from Ancient Bones and Teeth.
Ancient DNA
DNA extraction
Degraded DNA
Paleogenomics
Silica purification
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2019
2019
Historique:
entrez:
16
3
2019
pubmed:
16
3
2019
medline:
5
9
2019
Statut:
ppublish
Résumé
We provide a DNA extraction protocol optimized for the recovery of highly fragmented molecules preserved within bones and teeth. In this method, the hard tissue matrix is degraded using an EDTA/Proteinase K lysis buffer, and the DNA is purified using spin columns with silica membranes. This method efficiently recovers molecules as short as 35 base-pairs long.
Identifiants
pubmed: 30875041
doi: 10.1007/978-1-4939-9176-1_4
doi:
Substances chimiques
DNA, Ancient
0
Silicon Dioxide
7631-86-9
Edetic Acid
9G34HU7RV0
Endopeptidase K
EC 3.4.21.64
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM