Identification of the cytochrome P450 involved in the degradation of neonicotinoid insecticide acetamiprid in Phanerochaete chrysosporium.

Biodegradation, Environmental Cytochrome P-450 Enzyme System / metabolism Insecticides / metabolism Neonicotinoids / metabolism Phanerochaete / metabolism
Acetamiprid Cytochrome P450 Phanerochaete chrysosporium

Journal

Journal of hazardous materials
ISSN: 1873-3336
Titre abrégé: J Hazard Mater
Pays: Netherlands
ID NLM: 9422688

Informations de publication

Date de publication:
05 06 2019
Historique:
received: 10 11 2018
revised: 20 02 2019
accepted: 08 03 2019
pubmed: 16 3 2019
medline: 2 9 2020
entrez: 16 3 2019
Statut: ppublish

Résumé

We previously reported that cytochrome P450 s play critical roles in neonicotinoid insecticide biodegradation by white-rot fungi. Here, we investigated the biodegradation of acetamiprid (ACET) by Phanerochaete chrysosporium to identify the cytochrome P450 involved in this degradation process. During a 20-day incubation period, P. chrysosporium degraded 21% and 51% of ACET in ligninolytic and nonligninolytic media, respectively. The degradation rate of ACET was markedly decreased by the addition of cytochrome P450 inhibitors. Recombinant cytochrome P450s in P. chrysosporium (PcCYP) were heterologously expressed in Saccharomyces cerevisiae strain AH22, and the PcCYP involved in ACET degradation was identified. The results showed that CYP5147A3 can degrade ACET, and two ACET metabolites, N'-cyano-N-methyl acetamidine and 6-chloro-3-pyridinemethanol, were identified. To the best of our knowledge, this study provides the first characterization of the fungal cytochrome P450 that is responsible for the degradation and detoxification of ACET.

Identifiants

DOI: 10.1016/j.jhazmat.2019.03.042 PMID: 30875576
pubmed: 30875576
pii: S0304-3894(19)30303-6
doi: 10.1016/j.jhazmat.2019.03.042
pii:
doi:

Substances chimiques

Insecticides 0
Neonicotinoids 0
Cytochrome P-450 Enzyme System 9035-51-2

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

494-498

Informations de copyright

Copyright © 2019 Elsevier B.V. All rights reserved.

Auteurs

Jianqiao Wang (J)

Key Laboratory for Water Quality and Conservation of the Pearl River Delta, Ministry of Education, School of Environmental Science and Engineering, Guangzhou University, Guangzhou, 510006, China.

Haruka Ohno (H)

Faculty of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka, 422-8529, Japan.

Yuuri Ide (Y)

Faculty of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka, 422-8529, Japan.

Hirofumi Ichinose (H)

Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka, 812-8581, Japan.

Toshio Mori (T)

Faculty of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka, 422-8529, Japan.

Hirokazu Kawagishi (H)

Faculty of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka, 422-8529, Japan; Graduate School of Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka, 422-8529, Japan; Research Institute of Green Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka, 422-8529, Japan.

Hirofumi Hirai (H)

Faculty of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka, 422-8529, Japan; Research Institute of Green Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka, 422-8529, Japan. Electronic address: hirai.hirofumi@shizuoka.ac.jp.

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Classifications MeSH

questionsmedicales.fr › Environnement et santé publique › Santé publique › Pollution de l'environnement › Assainissement et restauration de l'environnement › Dépollution biologique de l'environnement › Identification of the cytochrome P450 involved...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Hémoprotéines › Cytochromes › Cytochrome P-450 enzyme system › Identification of the cytochrome P450 involved...
questionsmedicales.fr › Actions chimiques et utilisations › Actions toxiques › Pesticides › Insecticides › Identification of the cytochrome P450 involved...
questionsmedicales.fr › Composés hétérocycliques › Composés hétéromonocycliques › Néonicotinoïdes › Identification of the cytochrome P450 involved...
questionsmedicales.fr › Eucaryotes › Champignons › Basidiomycota › Polyporales › Phanerochaete › Identification of the cytochrome P450 involved...