Carriage meningococcal isolates with capsule null locus dominate among high school students in a non-endemic period, Italy, 2012-2013.
Adolescent
Anti-Bacterial Agents
/ pharmacology
Bacterial Capsules
/ genetics
Bacterial Proteins
/ genetics
Carrier State
/ epidemiology
DNA, Bacterial
/ genetics
Female
Genetic Variation
Genome, Bacterial
/ genetics
Genotype
Humans
Italy
/ epidemiology
Male
Meningococcal Infections
/ epidemiology
Microbial Sensitivity Tests
Multilocus Sequence Typing
Neisseria meningitidis
/ classification
Pharynx
/ microbiology
Phylogeny
Prevalence
Sequence Analysis, DNA
Serogroup
Students
Young Adult
Carriage
Neisseria meningitidis
Whole genome sequencing
fHbp
nadA
nhba
Journal
International journal of medical microbiology : IJMM
ISSN: 1618-0607
Titre abrégé: Int J Med Microbiol
Pays: Germany
ID NLM: 100898849
Informations de publication
Date de publication:
Historique:
received:
16
10
2018
revised:
19
02
2019
accepted:
05
03
2019
pubmed:
18
3
2019
medline:
5
9
2019
entrez:
18
3
2019
Statut:
ppublish
Résumé
Meningococcal disease incidence in Italy remains quite low in the overall population except for infants. Within a study on carriage isolates among high school students we aimed to define: i) the prevalence of carriage isolates, ii) the phenotypic and iii) the molecular features of meningococci by Whole Genome Sequencing (WGS). A total of 1697 pharyngeal samples from undergraduate students (age range 14-19 years) were collected from 2012 to 2013 from six larger cities in Italy. One hundred and twenty culture positive meningococci (7%) were analyzed. Carriage isolates were sent to the National Reference Laboratory for invasive meningococcal disease (IMD) for PCR-based serogroup identification, Multilocus Sequence Typing, PorA and FetA typing. Moreover, factor H binding protein (fHbp), Neisseria Heparin Binding Antigen (NHBA) and Neisserial adhesin A (NadA) were typed. Core genome MLST (cgMLST) was performed on a subsample of 75 carriage isolates. Capsule null locus (cnl) predominated (47%), followed by serogroup B (27%). The antimicrobial susceptibility profile revealed an high prevalence of reduced susceptibility to penicillin G (54%) and a full susceptibility to ceftriaxone, ciprofloxacin and rifampicin. Carriage isolates presented a high genetic diversity: the clonal complexes (cc
Identifiants
pubmed: 30878541
pii: S1438-4221(18)30528-9
doi: 10.1016/j.ijmm.2019.03.004
pii:
doi:
Substances chimiques
Anti-Bacterial Agents
0
Bacterial Proteins
0
DNA, Bacterial
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
182-188Informations de copyright
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