Immobilizing sulfotransferase 1A1 on magnetic microparticles and their evaluation using capillary electrophoresis.
Capillary electrophoresis
Immobilization
Magnetic microparticles
Paracetamol
Sulfotransferase
Journal
Electrophoresis
ISSN: 1522-2683
Titre abrégé: Electrophoresis
Pays: Germany
ID NLM: 8204476
Informations de publication
Date de publication:
09 2019
09 2019
Historique:
received:
10
01
2019
revised:
11
03
2019
accepted:
11
03
2019
pubmed:
19
3
2019
medline:
5
3
2020
entrez:
19
3
2019
Statut:
ppublish
Résumé
Sulfotransferases are categorized as phase II metabolic enzymes. Human sulfotransferase 1A1 (SULT1A1) is involved in the sulfonation of xenobiotics with aid from the cofactor 3'-phosphoadenosine-5'-phosphosulfate that acts as a sulfonate donor. In this study, we have attempted to immobilize SULT1A1 on magnetic microparticles (MMs). Different functionalized MMs were used to immobilize SULT1A1 and their enzyme activity was compared to the control (enzyme in solution). Paracetamol was used as model substrate. Separation of paracetamol and paracetamol sulfate by CE-UV was optimized and validated. MMs with epoxy based immobilization of SULT1A1 showed better enzyme activity. Hence, they were tested for repeated usage to allow their implementation for the development of a CE immobilized micro enzyme reactor.
Identifiants
pubmed: 30882918
doi: 10.1002/elps.201900016
doi:
Substances chimiques
Enzymes, Immobilized
0
Acetaminophen
362O9ITL9D
Arylsulfotransferase
EC 2.8.2.1
SULT1A1 protein, human
EC 2.8.2.1
acetaminophen sulfate ester
S6002H6J9F
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2271-2276Subventions
Organisme : FP7 Marie Skłodowska Curie
ID : 607517
Pays : International
Informations de copyright
© 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
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