Combined In-Cell NMR and Simulation Approach to Probe Redox-Dependent Pathway Control.

Cells, Cultured Hexoses / chemistry Kinetics Molecular Dynamics Simulation NAD / chemistry Nuclear Magnetic Resonance, Biomolecular Oxidation-Reduction Saccharomyces cerevisiae / cytology

Journal

Analytical chemistry
ISSN: 1520-6882
Titre abrégé: Anal Chem
Pays: United States
ID NLM: 0370536

Informations de publication

Date de publication:
16 04 2019
Historique:
pubmed: 22 3 2019
medline: 26 8 2020
entrez: 22 3 2019
Statut: ppublish

Résumé

Dynamic response of intracellular reaction cascades to changing environments is a hallmark of living systems. As metabolism is complex, mechanistic models have gained popularity for describing the dynamic response of cellular metabolism and for identifying target genes for engineering. At the same time, the detailed tracking of transient metabolism in living cells on the subminute time scale has become amenable using dynamic nuclear polarization-enhanced

Identifiants

DOI: 10.1021/acs.analchem.9b00660 PMID: 30896922
pubmed: 30896922
doi: 10.1021/acs.analchem.9b00660
doi:

Substances chimiques

Hexoses 0
NAD 0U46U6E8UK

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

5395-5402

Auteurs

Pernille R Jensen (PR)

Department of Chemistry, Department of Health Technology and The Novo Nordisk Foundation Center of Biosustainability , Technical University of Denmark , 2800 Kgs Lyngby , Denmark.
ORCID: 0000-0003-4359-848X

Marta R A Matos (MRA)

Department of Chemistry, Department of Health Technology and The Novo Nordisk Foundation Center of Biosustainability , Technical University of Denmark , 2800 Kgs Lyngby , Denmark.

Nikolaus Sonnenschein (N)

Department of Chemistry, Department of Health Technology and The Novo Nordisk Foundation Center of Biosustainability , Technical University of Denmark , 2800 Kgs Lyngby , Denmark.
ORCID: 0000-0002-7581-4936

Sebastian Meier (S)

Department of Chemistry, Department of Health Technology and The Novo Nordisk Foundation Center of Biosustainability , Technical University of Denmark , 2800 Kgs Lyngby , Denmark.
ORCID: 0000-0003-3761-3217

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Classifications MeSH

questionsmedicales.fr › Cellules › Cellules cultivées › Combined In-Cell NMR and Simulation Approach...
questionsmedicales.fr › Glucides › Sucres › Oses › Hexose › Combined In-Cell NMR and Simulation Approach...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Cinétique › Combined In-Cell NMR and Simulation Approach...
questionsmedicales.fr › Sciences de l'information › Méthodologies informatiques › Simulation numérique › Simulation de dynamique moléculaire › Combined In-Cell NMR and Simulation Approach...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Nucléotides › Ribonucléotides › Nucléotides adényliques › NAD › Combined In-Cell NMR and Simulation Approach...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Analyse spectrale › Spectroscopie par résonance magnétique › Résonance magnétique nucléaire biomoléculaire › Combined In-Cell NMR and Simulation Approach...
questionsmedicales.fr › Métabolisme › Métabolisme énergétique › Oxydoréduction › Combined In-Cell NMR and Simulation Approach...
questionsmedicales.fr › Eucaryotes › Champignons › Levures › Saccharomyces › Saccharomyces cerevisiae › Combined In-Cell NMR and Simulation Approach...