Factors influencing meiotic recombination revealed by whole-genome sequencing of single sperm.
Journal
Science (New York, N.Y.)
ISSN: 1095-9203
Titre abrégé: Science
Pays: United States
ID NLM: 0404511
Informations de publication
Date de publication:
22 03 2019
22 03 2019
Historique:
received:
27
07
2018
accepted:
01
02
2019
entrez:
23
3
2019
pubmed:
23
3
2019
medline:
24
8
2019
Statut:
ppublish
Résumé
Recombination is critical to meiosis and evolution, yet many aspects of the physical exchange of DNA via crossovers remain poorly understood. We report an approach for single-cell whole-genome DNA sequencing by which we sequenced 217 individual hybrid mouse sperm, providing a kilobase-resolution genome-wide map of crossovers. Combining this map with molecular assays measuring stages of recombination, we identified factors that affect crossover probability, including PRDM9 binding on the non-initiating template homolog and telomere proximity. These factors also influence the time for sites of recombination-initiating DNA double-strand breaks to find and engage their homologs, with rapidly engaging sites more likely to form crossovers. We show that chromatin environment on the template homolog affects positioning of crossover breakpoints. Our results also offer insights into recombination in the pseudoautosomal region.
Identifiants
pubmed: 30898902
pii: 363/6433/eaau8861
doi: 10.1126/science.aau8861
pmc: PMC6445350
mid: EMS82168
pii:
doi:
Substances chimiques
Chromatin
0
Histone-Lysine N-Methyltransferase
EC 2.1.1.43
prdm9 protein, mouse
EC 2.1.1.43
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Informations de copyright
Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.
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