Monitoring and control strategies for inclusion body production in E. coli based on glycerol consumption.

Escherichia coli / genetics Glycerol / chemistry Inclusion Bodies / drug effects Isopropyl Thiogalactoside / adverse effects Recombinant Proteins / biosynthesis
E. coli Glycerol Inclusion bodies Physiological process control Recombinant protein production

Journal

Journal of biotechnology
ISSN: 1873-4863
Titre abrégé: J Biotechnol
Pays: Netherlands
ID NLM: 8411927

Informations de publication

Date de publication:
20 Apr 2019
Historique:
received: 14 01 2019
revised: 12 03 2019
accepted: 18 03 2019
pubmed: 25 3 2019
medline: 28 7 2019
entrez: 25 3 2019
Statut: ppublish

Résumé

The Gram-negative bacterium E. coli is the host of choice for the production of a multitude of recombinant proteins in industry. Generally, cultivation is easy, media are cheap and a high product titer can be obtained. However, harsh induction procedures using IPTG as inducer are often referred to cause stress reactions, leading to a phenomenon known as metabolic burden and expression of inclusion bodies. In this contribution, we present different strategies for determination of critical timepoints for product stability in an E. coli IB bioprocess. As non-controlled feeding during induction regularly led to undesired product loss, we applied physiological feeding control. We found that the feeding strategy has indeed high impact on IB productivity. However, high applied q

Identifiants

DOI: 10.1016/j.jbiotec.2019.03.014 PMID: 30904592
pubmed: 30904592
pii: S0168-1656(19)30095-1
doi: 10.1016/j.jbiotec.2019.03.014
pii:
doi:

Substances chimiques

Recombinant Proteins 0
Isopropyl Thiogalactoside 367-93-1
Glycerol PDC6A3C0OX

Types de publication

Journal Article

Langues

eng

Pagination

75-82

Informations de copyright

Copyright © 2019 Elsevier B.V. All rights reserved.

Auteurs

Christoph Slouka (C)

Christian Doppler Laboratory for Mechanistic and Physiological Methods for Improved Bioprocesses, Institute of Chemical Environmental and Bioscience Engineering, Vienna University of Technology, Vienna, Austria. Electronic address: christoph.slouka@tuwien.ac.at.

Julian Kopp (J)

Christian Doppler Laboratory for Mechanistic and Physiological Methods for Improved Bioprocesses, Institute of Chemical Environmental and Bioscience Engineering, Vienna University of Technology, Vienna, Austria.

Daniel Strohmer (D)

Christian Doppler Laboratory for Mechanistic and Physiological Methods for Improved Bioprocesses, Institute of Chemical Environmental and Bioscience Engineering, Vienna University of Technology, Vienna, Austria.

Julian Kager (J)

Research Division Biochemical Engineering, Institute of Chemical Environmental and Bioscience Engineering, Vienna University of Technology, Vienna, Austria.

Oliver Spadiut (O)

Research Division Biochemical Engineering, Institute of Chemical Environmental and Bioscience Engineering, Vienna University of Technology, Vienna, Austria.

Christoph Herwig (C)

Christian Doppler Laboratory for Mechanistic and Physiological Methods for Improved Bioprocesses, Institute of Chemical Environmental and Bioscience Engineering, Vienna University of Technology, Vienna, Austria; Research Division Biochemical Engineering, Institute of Chemical Environmental and Bioscience Engineering, Vienna University of Technology, Vienna, Austria.

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Classifications MeSH

questionsmedicales.fr › Bactéries › Proteobacteria › Gammaproteobacteria › Enterobacteriaceae › Escherichia › Escherichia coli › Monitoring and control strategies for...
questionsmedicales.fr › Glucides › Polyols › Dérivés du glycérol › Glycérol › Monitoring and control strategies for...
questionsmedicales.fr › Cellules › Structures cellulaires › Corps d'inclusion › Monitoring and control strategies for...
questionsmedicales.fr › Glucides › Hétérosides › Thioglycosides › Thiogalactosides › Isopropyl-1-thio-bêta-D-galactopyranoside › Monitoring and control strategies for...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines recombinantes › Monitoring and control strategies for...