Chitosan and anisodamine improve the immune efficacy of inactivated infectious spleen and kidney necrosis virus vaccine in Siniperca chuatsi.
Adjuvants, Immunologic
/ administration & dosage
Animals
Chitosan
/ immunology
DNA Virus Infections
/ immunology
Enzymes
/ blood
Fish Diseases
/ immunology
Fish Proteins
/ genetics
Gene Expression Profiling
/ veterinary
Immunity, Innate
/ drug effects
Iridoviridae
/ immunology
Perciformes
/ genetics
Poly I-C
/ immunology
Solanaceous Alkaloids
/ immunology
Viral Vaccines
/ immunology
Virus Replication
/ drug effects
Adjuvant
Anisodamine
Chitosan
ISKNV
Siniperca chuatsi
ims1312
poly(I:C)
Journal
Fish & shellfish immunology
ISSN: 1095-9947
Titre abrégé: Fish Shellfish Immunol
Pays: England
ID NLM: 9505220
Informations de publication
Date de publication:
Jun 2019
Jun 2019
Historique:
received:
14
01
2019
revised:
14
03
2019
accepted:
15
03
2019
pubmed:
25
3
2019
medline:
25
7
2019
entrez:
25
3
2019
Statut:
ppublish
Résumé
Siniperca chuatsi is an economically important fish in China, but infectious spleen and kidney necrosis virus (ISKNV) causes high mortality and significant economic losses. Currently, vaccination is the most promising strategy to prevent infectious diseases, while adjuvant can effectively enhance immune responses. In this study, inactivated ISKNV vaccine was prepared, then poly (I:C), chitosan, anisodamine and ims1312 were used as adjuvants to evaluate the effect on the immune responses and ISKNV replication. Chitosan could strongly boost the protection of liver and spleen tissues by pathological sections. In serum, poly (I:C) and chitosan group had protective effect on catalase, acid phosphatase, blood urea nitrogen. mRNA expressions showed these adjuvants induced the cytokines of early immune responses (TNF-α, Viperin) in both spleen and mesonephron by real time quantitative RT-PCR assays. Meanwhile, poly (I:C), chitosan and anisodamine were significantly improved the antiviral function and inhibited ISKNV replication. Chitosan and anisodamine played a significantly protective role in the immune protective rate test. The results indicated that all the four adjuvants are valid in the inactivated ISKNV vaccine, and chitosan is recommended preferentially. The present study provides reference for other animal vaccine adjuvants.
Identifiants
pubmed: 30904683
pii: S1050-4648(19)30188-3
doi: 10.1016/j.fsi.2019.03.040
pii:
doi:
Substances chimiques
Adjuvants, Immunologic
0
Enzymes
0
Fish Proteins
0
Solanaceous Alkaloids
0
Viral Vaccines
0
anisodamine
01343Q8EL8
Chitosan
9012-76-4
Poly I-C
O84C90HH2L
Types de publication
Journal Article
Langues
eng
Pagination
52-60Informations de copyright
Copyright © 2019 Elsevier Ltd. All rights reserved.