FM19G11-Loaded Gold Nanoparticles Enhance the Proliferation and Self-Renewal of Ependymal Stem Progenitor Cells Derived from ALS Mice.
Amyotrophic Lateral Sclerosis
Animals
Benzamides
/ pharmacology
Biomarkers
/ metabolism
Cell Cycle
/ drug effects
Cell Proliferation
/ drug effects
Cell Self Renewal
/ drug effects
Ependyma
/ cytology
Gene Expression Regulation
/ drug effects
Gold
/ chemistry
Humans
Metal Nanoparticles
/ chemistry
Mice, Transgenic
MicroRNAs
/ genetics
Octamer Transcription Factor-3
/ metabolism
PTEN Phosphohydrolase
/ metabolism
Pluripotent Stem Cells
/ metabolism
Proto-Oncogene Proteins c-akt
/ metabolism
SOXB1 Transcription Factors
/ metabolism
Stem Cells
/ cytology
Superoxide Dismutase-1
/ metabolism
Uncoupling Protein 2
/ metabolism
FM19G11
G93A-SOD1 mouse model
amyotrophic lateral sclerosis
ependymal stem progenitor cells
nanoparticles
Journal
Cells
ISSN: 2073-4409
Titre abrégé: Cells
Pays: Switzerland
ID NLM: 101600052
Informations de publication
Date de publication:
23 03 2019
23 03 2019
Historique:
received:
21
02
2019
revised:
18
03
2019
accepted:
20
03
2019
entrez:
27
3
2019
pubmed:
27
3
2019
medline:
27
3
2019
Statut:
epublish
Résumé
Amyotrophic lateral sclerosis (ALS) is a progressive neurodegenerative disease affecting motor neurons. In ALS mice, neurodegeneration is associated with the proliferative restorative attempts of ependymal stem progenitor cells (epSPCs) that normally lie in a quiescent in the spinal cord. Thus, modulation of the proliferation of epSPCs may represent a potential strategy to counteract neurodegeneration. Recent studies demonstrated that FM19G11, a hypoxia-inducible factor modulator, induces epSPC self-renewal and proliferation. The aim of the study was to investigate whether FM19G11-loaded gold nanoparticles (NPs) can affect self-renewal and proliferation processes in epSPCs isolated from G93A-SOD1 mice at disease onset. We discovered elevated levels of SOX2, OCT4, AKT1, and AKT3, key genes associated with pluripotency, self-renewal, and proliferation, in G93A-SOD1 epSPCs at the transcriptional and protein levels after treatment with FM19G11-loaded NPs. We also observed an increase in the levels of the mitochondrial uncoupling protein (
Identifiants
pubmed: 30909571
pii: cells8030279
doi: 10.3390/cells8030279
pmc: PMC6468696
pii:
doi:
Substances chimiques
Benzamides
0
Biomarkers
0
FM19G11
0
MicroRNAs
0
Octamer Transcription Factor-3
0
Pou5f1 protein, mouse
0
SOXB1 Transcription Factors
0
Uncoupling Protein 2
0
Gold
7440-57-5
Superoxide Dismutase-1
EC 1.15.1.1
Proto-Oncogene Proteins c-akt
EC 2.7.11.1
PTEN Phosphohydrolase
EC 3.1.3.67
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
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