Sequence and expression levels of circular RNAs in progenitor cell types during mouse corticogenesis.
Alternative Splicing
/ genetics
Animals
Base Sequence
/ genetics
Binding Sites
Cerebellar Cortex
/ cytology
Exons
/ genetics
Female
Mice
Mice, Transgenic
MicroRNAs
/ genetics
Neurogenesis
/ genetics
Pregnancy
Protein Biosynthesis
/ genetics
RNA, Circular
/ genetics
RNA, Messenger
/ genetics
Stem Cells
/ physiology
Transcriptome
/ genetics
Journal
Life science alliance
ISSN: 2575-1077
Titre abrégé: Life Sci Alliance
Pays: United States
ID NLM: 101728869
Informations de publication
Date de publication:
04 2019
04 2019
Historique:
received:
21
02
2019
revised:
22
03
2019
accepted:
22
03
2019
entrez:
31
3
2019
pubmed:
31
3
2019
medline:
31
3
2019
Statut:
epublish
Résumé
Circular (circ) RNAs have recently emerged as a novel class of transcripts whose identification and function remain elusive. Among many tissues and species, the mammalian brain is the organ in which circRNAs are more abundant and first evidence of their functional significance started to emerge. Yet, even within this well-studied organ, annotation of circRNAs remains fragmentary, their sequence is unknown, and their expression in specific cell types was never investigated. Overcoming these limitations, here we provide the first comprehensive identification of circRNAs and assessment of their expression patterns in proliferating neural stem cells, neurogenic progenitors, and newborn neurons of the developing mouse cortex. Extending the current knowledge about the diversity of this class of transcripts by the identification of nearly 4,000 new circRNAs, our study is the first to provide the full sequence information and expression patterns of circRNAs in cell types representing the lineage of neurogenic commitment. We further exploited our data by evaluating the coding potential, evolutionary conservation, and biogenesis of circRNAs that we found to arise from a specific subclass of linear mRNAs. Our study provides the arising field of circRNA biology with a powerful new resource to address the complexity and potential biological significance of this new class of transcripts.
Identifiants
pubmed: 30926618
pii: 2/2/e201900354
doi: 10.26508/lsa.201900354
pmc: PMC6441494
pii:
doi:
Substances chimiques
MicroRNAs
0
RNA, Circular
0
RNA, Messenger
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© 2019 Dori et al.
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