Enzyme Kinetics Determined by Single-Injection Isothermal Titration Calorimetry.

Calorimetry / methods Catalysis Enzymes / chemistry Hot Temperature Kinetics Thermodynamics
Calorimetry Enthalpy Enzyme ITC Kinetics Michaelis-Menten Single-injection method

Journal

Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969

Informations de publication

Date de publication:
2019
Historique:
entrez: 1 4 2019
pubmed: 1 4 2019
medline: 2 8 2019
Statut: ppublish

Résumé

This chapter describes how to collect Michaelis-Menten kinetic data on an enzyme-catalyzed reaction with the isothermal titration calorimetry (ITC) and the single-injection method. ITC measures the heat rate which is directly proportional to the reaction rate. The enthalpy change (Δ

Identifiants

DOI: 10.1007/978-1-4939-9179-2_17 PMID: 30929247
pubmed: 30929247
doi: 10.1007/978-1-4939-9179-2_17
doi:

Substances chimiques

Enzymes 0

Types de publication

Journal Article

Langues

eng

Pagination

241-249

Auteurs

Colette F Quinn (CF)

Applications Lab, TA Instruments, Lindon, UT, USA. cquinn@tainstruments.com.

Lee D Hansen (LD)

Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT, USA.

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Classifications MeSH

questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Calorimétrie › Enzyme Kinetics Determined by Single-Injection...
questionsmedicales.fr › Phénomènes chimiques › Catalyse › Enzyme Kinetics Determined by Single-Injection...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Enzyme Kinetics Determined by Single-Injection...
questionsmedicales.fr › Environnement et santé publique › Environnement › Concepts météorologiques › Atmosphère › Temps (météorologie) › Température › Température élevée › Enzyme Kinetics Determined by Single-Injection...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Cinétique › Enzyme Kinetics Determined by Single-Injection...
questionsmedicales.fr › Phénomènes physiques › Thermodynamique › Enzyme Kinetics Determined by Single-Injection...