Experimental and numerical study of band-broadening effects associated with analyte transfer in microfluidic devices for spatial two-dimensional liquid chromatography created by additive manufacturing.
Additive manufacturing
Analyte transfer
Band broadening
Computational fluid dynamics
LC × LC
Spatial chromatography
Journal
Journal of chromatography. A
ISSN: 1873-3778
Titre abrégé: J Chromatogr A
Pays: Netherlands
ID NLM: 9318488
Informations de publication
Date de publication:
02 Aug 2019
02 Aug 2019
Historique:
received:
30
11
2018
revised:
19
03
2019
accepted:
20
03
2019
pubmed:
2
4
2019
medline:
30
6
2019
entrez:
2
4
2019
Statut:
ppublish
Résumé
Conventional one-dimensional column-based liquid chromatographic (LC) systems do not offer sufficient separation power for the analysis of complex mixtures. Column-based comprehensive two-dimensional liquid chromatography offers a higher separation power, yet suffers from instrumental complexity and long analysis times. Spatial two-dimensional liquid chromatography can be considered as an alternative to column-based approaches. The peak capacity of the system is ideally the product of the peak capacities of the two dimensions, yet the analysis time remains relatively short due to parallel second-dimension separations. Aspects affecting the separation efficiency of this type of systems include flow distribution to homogeneously distribute the mobile phase for the second-dimension (
Identifiants
pubmed: 30929867
pii: S0021-9673(19)30309-7
doi: 10.1016/j.chroma.2019.03.041
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
77-84Informations de copyright
Copyright © 2019 The Author(s). Published by Elsevier B.V. All rights reserved.