Leukocyte Cytoskeleton Polarization Is Initiated by Plasma Membrane Curvature from Cell Attachment.
Actins
/ metabolism
Animals
Cell Adhesion
Cell Membrane
/ metabolism
Cell Movement
/ physiology
Cell Polarity
/ physiology
Cell-Matrix Junctions
Cytoskeleton
/ metabolism
Endothelium
/ metabolism
Female
GTPase-Activating Proteins
/ metabolism
HEK293 Cells
Humans
Leukocytes
/ physiology
Male
Mice
Mice, Inbred C57BL
Mice, Knockout
Minor Histocompatibility Antigens
/ metabolism
Myosin Light Chains
/ metabolism
Neutrophils
/ metabolism
Phosphatidylinositol Phosphates
/ metabolism
Phosphorylation
Phosphotransferases (Alcohol Group Acceptor)
/ metabolism
Signal Transduction
BAR domain
SRGAP2
adhesion
cell polarization
neutrophil
phosphatidylinositol-4-phosphate
Journal
Developmental cell
ISSN: 1878-1551
Titre abrégé: Dev Cell
Pays: United States
ID NLM: 101120028
Informations de publication
Date de publication:
22 04 2019
22 04 2019
Historique:
received:
15
03
2018
revised:
15
01
2019
accepted:
25
02
2019
pubmed:
2
4
2019
medline:
7
1
2020
entrez:
2
4
2019
Statut:
ppublish
Résumé
Cell polarization is important for various biological processes. However, its regulation, particularly initiation, is incompletely understood. Here, we investigated mechanisms by which neutrophils break their symmetry and initiate their cytoskeleton polarization from an apolar state in circulation for their extravasation during inflammation. We show here that a local increase in plasma membrane (PM) curvature resulting from cell contact to a surface triggers the initial breakage of the symmetry of an apolar neutrophil and is required for subsequent polarization events induced by chemical stimulation. This local increase in PM curvature recruits SRGAP2 via its F-BAR domain, which in turn activates PI4KA and results in PM PtdIns4P polarization. Polarized PM PtdIns4P is targeted by RPH3A, which directs PIP5K1C90 and subsequent phosphorylated myosin light chain polarization, and this polarization signaling axis regulates neutrophil firm attachment to endothelium. Thus, this study reveals a mechanism for the initiation of cell cytoskeleton polarization.
Identifiants
pubmed: 30930167
pii: S1534-5807(19)30146-7
doi: 10.1016/j.devcel.2019.02.023
pmc: PMC6482112
mid: NIHMS1525314
pii:
doi:
Substances chimiques
Actins
0
GTPase-Activating Proteins
0
Minor Histocompatibility Antigens
0
Myosin Light Chains
0
Phosphatidylinositol Phosphates
0
SRGAP2 protein, human
0
phosphatidylinositol 4-phosphate
0
Phosphotransferases (Alcohol Group Acceptor)
EC 2.7.1.-
phosphatidylinositol phosphate 4-kinase
EC 2.7.1.67
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
206-219.e7Subventions
Organisme : NCATS NIH HHS
ID : UL1 TR001863
Pays : United States
Organisme : NHLBI NIH HHS
ID : R35 HL135805
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL125885
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM108954
Pays : United States
Organisme : NINDS NIH HHS
ID : R01 NS113236
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM114513
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL145152
Pays : United States
Informations de copyright
Copyright © 2019 Elsevier Inc. All rights reserved.
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