Optimization of continuous purification of recombinant patchoulol synthase from Escherichia coli with membrane adsorbers.
membrane chromatography
patchouli oil
patchoulol synthase
periodic counter-current chromatography
sesquiterpenes
Journal
Biotechnology progress
ISSN: 1520-6033
Titre abrégé: Biotechnol Prog
Pays: United States
ID NLM: 8506292
Informations de publication
Date de publication:
07 2019
07 2019
Historique:
received:
14
01
2019
revised:
13
03
2019
accepted:
21
03
2019
pubmed:
2
4
2019
medline:
2
6
2020
entrez:
2
4
2019
Statut:
ppublish
Résumé
The natural production of patchouli oil in developing countries cannot meet the increasing demand any more. This leads to socioecological consequences, such as the use of arable land, which is actually intended for food. Hence, the world market price increased up to $150/kg. An alternative is the biotechnological production of patchouli oil using a multiproduct sesquiterpene synthase, the patchoulol synthase (PTS). Here, we report the optimization of recombinant PTS purification from Escherichia coli lysate using continuous immobilized metal affinity chromatography. First, the purification conditions of the batch process were optimized in regard to optimal buffer composition and optimized chromatographic conditions. The best purification result was achieved with Co
Substances chimiques
Recombinant Proteins
0
Isomerases
EC 5.-
patchoulol synthase
EC 5.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2812Informations de copyright
© 2019 American Institute of Chemical Engineers.
Références
Buchi G, Macleod WD. Synthesis of patchouli alcohol. J Am Chem Soc. 1962;84(16):3205-3206. https://doi.org/10.1021/ja00875a047.
Deguerry F, Pastore L, Wu S, Clark A, Chappell J, Schalk M. The diverse sesquiterpene profile of patchouli, Pogostemon cablin, is correlated with a limited number of sesquiterpene synthases. Arch Biochem Biophys. 2006;454(2):123-136. https://doi.org/10.1016/j.abb.2006.08.006.
Badan Standarisasi Nasional. Standar Nasional Indonesia, Minyak Nilam. SNI 06-2385-2006, Jakarta; 2006.
Organisation Internationale de Normalisation. Huile essentielle de patchouli (Pogostemon cablin [Blanco] Benth.); 2002.
Maxwell S, Fernando A. Cash crops in developing countries: the issues, the facts, the policies. World Dev. 1989;17(11):1677-1708.
Frister T, Beutel S. Moschusduft und Patchouliöl. Chem Unserer Zeit. 2015;49(5):294-301.
Insights FM. Patchouli Oil Sales Revenue to Surpass US$ 67 Million by 2019 - Future Market Insights. https://globenewswire.com/news-release/2018/12/10/1664400/0/en/Patchouli-Oil-Sales-Revenue-to-Surpass-US-67-Million-by-2019-Future-Market-Insights.html. Published 2018. Accessed February 27, 2019.
Henke NA, Wichmann J, Baier T, et al. Patchoulol production with metabolically engineered Corynebacterium glutamicum. Genes (Basel). 2018;9(4):219.
Xu G-Q, Lin G-Q, Sun B-F. Concise asymmetric total synthesis of (−)-patchouli alcohol. Org Chem Front. 2017;4(10):2031-2033.
Kusuma HS, Mahfud M. The extraction of essential oils from patchouli leaves (Pogostemon cablin Benth) using a microwave air-hydrodistillation method as a new green technique. RSC Adv. 2017;7(3):1336-1347. https://doi.org/10.1039/C6RA25894H.
Bohlmann J, Keeling CI. Terpenoid biomaterials. Plant J. 2008;54(4):656-669.
Daviet L, Schalk M. Biotechnology in plant essential oil production: progress and perspective in metabolic engineering of the terpene pathway. Flavour Fragr J. 2010;25(3):123-127.
Asadollahi MA, Maury J, Møller K, et al. Production of plant sesquiterpenes in Saccharomyces cerevisiae: effect of ERG9 repression on sesquiterpene biosynthesis. Biotechnol Bioeng. 2008;99(3):666-677.
Clearwood® 970953. http://www.firmenich.com/uploads/files/ingredients/marketing-sheet/perfumery/CLEARWOOD_970953.pdf. Accessed October 11, 2018.
Ohloff G, Pickenhagen W, Kraft P. Scent and Chemistry. Zürich: Verlag Helvetica Chimica Acta; 2011.
Hartwig S, Frister T, Alemdar S, et al. Expression, purification and activity assay of a patchoulol synthase cDNA variant fused to thioredoxin in Escherichia coli. Protein Expr Purif. 2014;97:61-71. https://doi.org/10.1016/j.pep.2014.02.003.
Frister T, Hartwig S, Alemdar S, et al. Characterisation of a recombinant patchoulol synthase variant for biocatalytic production of Terpenes. Appl Biochem Biotechnol. 2015;176(8):2185-2201. https://doi.org/10.1007/s12010-015-1707-y.
Munck SL, Croteau R. Purification and characterization of the sesquiterpene cyclase patchoulol synthase from Pogostemon cablin. Arch Biochem Biophys. 1990;282(1):58-64. https://doi.org/10.1016/0003-9861(90)90086-E.
Buzek J, Ask B. Regulation (EC) No 1223/2009 of the European Parliament and of the Council of 30 November 2009 on cosmetic products. Off J Eur Union L. 2009;342.
Federal Food, Drug, and Cosmetic Act (FD&C Act). https://www.fda.gov/regulatoryinformation/lawsenforcedbyfda/federalfooddrugandcosmeticactfdcact/default.htm. Accessed January 9, 2019.
EFfCI GMP FOR COSMETIC INGREDIENTS Including the Certification Scheme for GMP for Cosmetic Ingredients Revision 2017 Prepared by the European Federation for Cosmetic Ingredients In Collaboration with; 2017.
Knudsen HL, Fahrner RL, Xu Y, Norling LA, Blank GS. Membrane ion-exchange chromatography for process-scale antibody purification. J Chromatogr A. 2001;907(1):145-154.
Wang J. Macroporous ion-exchange membrane adsorbers: correlation between membrane structure, separation conditions and performance in bioseparation; 2009.
Tennikova TB, Svec F. High-performance membrane chromatography: highly efficient separation method for proteins in ion-exchange, hydrophobic interaction and reversed-phase modes. J Chromatogr A. 1993;646(2):279-288.
Demmer W, Nussbaumer D. Large-scale membrane adsorbers. J Chromatogr A. 1999;852(1):73-81.
Chatterjee S. FDA perspective on continuous manufacturing. IFPAC Annual Meeting, Baltimore, MD; 2012.
Godawat R, Brower K, Jain S, Konstantinov K, Riske F, Warikoo V. Periodic counter-current chromatography-design and operational considerations for integrated and continuous purification of proteins. Biotechnol J. 2012;7(12):1496-1508.
Mahajan E, George A, Wolk B. Improving affinity chromatography resin efficiency using semi-continuous chromatography. J Chromatogr A. 2012;1227:154-162.
Castan A, Falkman T, Faldt E, Persson T, Blomqvist L, Forss A. Process intensification through integration of upstream perfusion cell culture with downstream continuous chromatography in monoclonal antibody production; 2016.
Gjoka X, Gantier R, Schofield M. Transfer of a three step mAb chromatography process from batch to continuous: optimizing productivity to minimize consumable requirements. J Biotechnol. 2017;242:11-18.
Brämer C, Schreiber S, Scheper T, Beutel S. Continuous purification of Candida antarctica lipase B using 3-membrane adsorber periodic counter-current chromatography. Eng Life Sci. 2018;18(7):414-424.
Gibson DG, Young L, Chuang R-Y, Venter JC, Hutchison CA, Smith HO. Enzymatic assembly of DNA molecules up to several hundred kilobases. Nat Methods. 2009;6(5):343-345. https://doi.org/10.1038/nmeth.1318.
Hartwig S. Biokatalytische Wege zur Darstellung funktioneller Sesquiterpene; 2016.
Kaltenbrunner O, Diaz L, Hu X, Shearer M. Continuous bind-and-elute protein A capture chromatography: optimization under process scale column constraints and comparison to batch operation. Biotechnol Prog. 2016;32(4):938-948. https://doi.org/10.1002/btpr.2291.
Ferreira CMH, Pinto ISS, Soares EV, Soares HMVM. (Un)suitability of the use of pH buffers in biological, biochemical and environmental studies and their interaction with metal ions - a review. RSC Adv. 2015;5(39):30989-31003. https://doi.org/10.1039/C4RA15453C.
GE Healthcare. Affinity Chromatography, Vol. 2 Tagged Proteins. Vol 2. Uppsala Sweden: GE Healthcare Bio-Sciences AB; 2017:1-284.
Christianson DW. Structural biology and chemistry of the terpenoid cyclases. Chem Rev. 2006;106(8):3412-3442. https://doi.org/10.1021/cr050286w.
Vagenende V, Yap MGS, Trout BL. Mechanisms of protein stabilization and prevention of protein aggregation by glycerol. Biochemistry. 2009;48(46):11084-11096. https://doi.org/10.1021/bi900649t.
Reed G, Nagodawithana TW. Biotechnology: Enzymes, Biomass, Food and Feed. Vol 9. Weinheim, VCH; 1995.
Janson J-C. Protein Purification: Principles, High Resolution Methods, and Applications. Vol 151. Somerset: John Wiley & Sons; 2012.
Cazes J. Encyclopedia of Chromatography. Vol 2. Boca Raton: CRC Press; 2005.
Frister THW. Herstellung, Charakterisierung und Anwendung einer rekombinanten Patchoulolsynthase zur biokatalytischen Herstellung von Sesquiterpenen; 2015.
Williams KL. Endotoxins: Pyrogens, LAL Testing and Depyrogenation. Boca Raton: CRC Press; 2007.
Affairs O of R. Inspection Technical Guides - Bacterial Endotoxins/Pyrogens. https://www.fda.gov/iceci/inspections/inspectionguides/inspectiontechnicalguides/ucm072918.htm. Accessed February 28, 2019.
Pollock J, Bolton G, Coffman J, Ho SV, Bracewell DG, Farid SS. Optimising the design and operation of semi-continuous affinity chromatography for clinical and commercial manufacture. J Chromatogr A. 2013;1284:17-27.