Binding of IFT22 to the intraflagellar transport complex is essential for flagellum assembly.
Trypanosoma brucei
GTPase
IFT22
cilia
intraflagellar transport
Journal
The EMBO journal
ISSN: 1460-2075
Titre abrégé: EMBO J
Pays: England
ID NLM: 8208664
Informations de publication
Date de publication:
02 05 2019
02 05 2019
Historique:
received:
04
12
2018
revised:
28
02
2019
accepted:
04
03
2019
pubmed:
4
4
2019
medline:
7
1
2020
entrez:
4
4
2019
Statut:
ppublish
Résumé
Intraflagellar transport (IFT) relies on motor proteins and the IFT complex to construct cilia and flagella. The IFT complex subunit IFT22/RabL5 has sequence similarity with small GTPases although the nucleotide specificity is unclear because of non-conserved G4/G5 motifs. We show that IFT22 specifically associates with G-nucleotides and present crystal structures of IFT22 in complex with GDP, GTP, and with IFT74/81. Our structural analysis unravels an unusual GTP/GDP-binding mode of IFT22 bypassing the classical G4 motif. The GTPase switch regions of IFT22 become ordered upon complex formation with IFT74/81 and mediate most of the IFT22-74/81 interactions. Structure-based mutagenesis reveals that association of IFT22 with the IFT complex is essential for flagellum construction in
Identifiants
pubmed: 30940671
pii: embj.2018101251
doi: 10.15252/embj.2018101251
pmc: PMC6484408
pii:
doi:
Substances chimiques
Protozoan Proteins
0
Guanosine Diphosphate
146-91-8
Guanosine Triphosphate
86-01-1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© 2019 The Authors.
Références
J Cell Biol. 1999 Nov 1;147(3):519-30
pubmed: 10545497
Curr Biol. 2005 Oct 25;15(20):1861-6
pubmed: 16243034
Nat Commun. 2015 Jun 16;6:7092
pubmed: 26077881
Cell. 2009 May 29;137(5):938-48
pubmed: 19490898
Acta Crystallogr D Biol Crystallogr. 2010 Feb;66(Pt 2):125-32
pubmed: 20124692
J Cell Sci. 2019 Feb 22;132(5):
pubmed: 30709917
Science. 2001 Nov 9;294(5545):1299-304
pubmed: 11701921
Methods Mol Biol. 2016;1454:69-82
pubmed: 27514916
Acta Crystallogr D Biol Crystallogr. 2011 Apr;67(Pt 4):235-42
pubmed: 21460441
Nature. 2010 Oct 14;467(7317):805-10
pubmed: 20944740
Am J Hum Genet. 2011 Apr 8;88(4):508-15
pubmed: 21473986
Mol Biochem Parasitol. 2007 Jun;153(2):220-3
pubmed: 17408766
Biol Reprod. 2017 May 1;96(5):993-1006
pubmed: 28430876
Mol Syst Biol. 2011 Oct 11;7:539
pubmed: 21988835
J Cell Sci. 2006 May 1;119(Pt 9):1852-63
pubmed: 16608878
Cytoskeleton (Hoboken). 2012 Jan;69(1):33-48
pubmed: 22076686
J Cell Biol. 1999 Feb 8;144(3):473-81
pubmed: 9971742
Proc Natl Acad Sci U S A. 1993 Jun 15;90(12):5519-23
pubmed: 8516294
Mol Biol Cell. 2008 Mar;19(3):929-44
pubmed: 18094047
J Biol Chem. 2011 Jul 29;286(30):26344-52
pubmed: 21642430
Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):6943-8
pubmed: 23569277
J Biol Chem. 2000 Dec 22;275(51):40174-9
pubmed: 11013266
Proc Natl Acad Sci U S A. 2005 Apr 19;102(16):5685-90
pubmed: 15821030
J Cell Biol. 1998 May 18;141(4):993-1008
pubmed: 9585417
Nucleic Acids Res. 2010 Jan;38(Database issue):D457-62
pubmed: 19843604
Nat Rev Mol Cell Biol. 2002 Nov;3(11):813-25
pubmed: 12415299
Curr Biol. 2007 Feb 6;17(3):193-202
pubmed: 17276912
Dev Cell. 2014 Nov 10;31(3):279-290
pubmed: 25446516
Nucleic Acids Res. 2005 Jul 1;33(Web Server issue):W299-302
pubmed: 15980475
Mol Cell. 2007 Sep 21;27(6):976-91
pubmed: 17889670
J Biol Chem. 2003 Aug 1;278(31):29336-43
pubmed: 12732635
Nature. 1993 Nov 18;366(6452):268-70
pubmed: 8232586
Cilia. 2013 Aug 14;2(1):10
pubmed: 23945166
Mol Microbiol. 2010 Oct;78(1):173-86
pubmed: 20923419
J Cell Biol. 2005 Jul 4;170(1):103-13
pubmed: 15998802
J Cell Sci. 2010 Feb 15;123(Pt 4):529-36
pubmed: 20145001
Science. 2013 Aug 30;341(6149):1009-12
pubmed: 23990561
J Biol Chem. 1996 Aug 23;271(34):20470-8
pubmed: 8702787
Small GTPases. 2018 Mar 4;9(1-2):76-94
pubmed: 29072526
Elife. 2014 Jan 01;3:e01566
pubmed: 24596149
Nat Struct Mol Biol. 2014 Dec;21(12):1035-41
pubmed: 25402481
Acta Crystallogr D Biol Crystallogr. 2010 Apr;66(Pt 4):486-501
pubmed: 20383002
EMBO J. 2011 May 18;30(10):1907-18
pubmed: 21505417
J Cell Sci. 2006 Oct 1;119(Pt 19):4088-100
pubmed: 16968739
J Cell Sci. 2009 Mar 15;122(Pt 6):834-41
pubmed: 19240117
Biol Open. 2015 Aug 14;4(9):1143-53
pubmed: 26276100
Sci Rep. 2016 Sep 26;6:34232
pubmed: 27666822
Proc Natl Acad Sci U S A. 1997 Apr 29;94(9):4457-62
pubmed: 9114011
Cell Microbiol. 2014 Mar;16(3):425-33
pubmed: 24134537
Differentiation. 2012 Feb;83(2):S12-22
pubmed: 22118932
EMBO J. 2016 Apr 1;35(7):773-90
pubmed: 26912722
Acta Crystallogr D Biol Crystallogr. 2010 Feb;66(Pt 2):213-21
pubmed: 20124702
J Cell Sci. 1993 Aug;105 ( Pt 4):1101-13
pubmed: 8227199
Semin Cell Dev Biol. 2011 Feb;22(1):48-56
pubmed: 20951823
J Eukaryot Microbiol. 1999 Mar-Apr;46(2):105-9
pubmed: 10361731
Nature. 2003 Nov 6;426(6962):83-7
pubmed: 14603322
Mol Biol Cell. 2015 Dec 1;26(24):4358-72
pubmed: 26424803
PLoS Genet. 2012;8(10):e1002969
pubmed: 23055941
Elife. 2014 Apr 24;3:e02419
pubmed: 24843028
Cell. 2008 May 2;133(3):427-39
pubmed: 18455984
Nat Rev Mol Cell Biol. 2009 Aug;10(8):513-25
pubmed: 19603039
Annu Rev Microbiol. 2009;63:335-62
pubmed: 19575562
Nucleic Acids Res. 2016 Jul 8;44(W1):W410-5
pubmed: 27131380
Biochemistry. 1995 Jan 17;34(2):593-9
pubmed: 7819254
Acta Crystallogr D Biol Crystallogr. 2004 Mar;60(Pt 3):432-8
pubmed: 14993666
Mol Biol Cell. 1999 Mar;10(3):693-712
pubmed: 10069812
Mol Biol Cell. 2017 Jun 15;28(12):1652-1666
pubmed: 28428259
Dev Cell. 2012 May 15;22(5):940-51
pubmed: 22595669
J Cell Biol. 2014 Oct 27;207(2):269-82
pubmed: 25349261
Am J Hum Genet. 2009 May;84(5):706-11
pubmed: 19442771
EMBO J. 2003 Oct 15;22(20):5336-46
pubmed: 14532107
Nat Rev Microbiol. 2014 Jul;12(7):505-18
pubmed: 24931043
J Cell Sci. 2016 Aug 1;129(15):3026-41
pubmed: 27343245
Nat Rev Mol Cell Biol. 2017 Sep;18(9):533-547
pubmed: 28698599
Acta Trop. 1979 Sep;36(3):289-92
pubmed: 43092
Nat Cell Biol. 2015 Dec;17(12):1536-45
pubmed: 26523365
J Cell Biol. 2000 Oct 30;151(3):709-18
pubmed: 11062270
J Mol Biol. 1993 Sep 5;233(1):123-38
pubmed: 8377180
J Struct Biol. 2018 Apr;202(1):51-60
pubmed: 29248600
Cell Motil Cytoskeleton. 2009 Aug;66(8):457-68
pubmed: 19253336
J Cell Biol. 2017 May 1;216(5):1489-1499
pubmed: 28381413
Acta Biochim Pol. 2001;48(4):829-50
pubmed: 11995995
J Biol Chem. 2005 Jul 29;280(30):27688-96
pubmed: 15955805
Dev Cell. 2017 Jul 10;42(1):22-36.e12
pubmed: 28625565
Dev Biol. 2017 Dec 1;432(1):125-139
pubmed: 28964737
Mol Biol Cell. 2006 Dec;17(12):5053-62
pubmed: 17021254
Cell Mol Life Sci. 2005 Dec;62(24):3014-38
pubmed: 16314935