Global Characterization of X Chromosome Inactivation in Human Pluripotent Stem Cells.
X inactivation
XIST
human embryonic stem cells
human induced pluripotent stem cells
Journal
Cell reports
ISSN: 2211-1247
Titre abrégé: Cell Rep
Pays: United States
ID NLM: 101573691
Informations de publication
Date de publication:
02 04 2019
02 04 2019
Historique:
received:
27
09
2018
revised:
15
01
2019
accepted:
05
03
2019
entrez:
4
4
2019
pubmed:
4
4
2019
medline:
2
6
2020
Statut:
ppublish
Résumé
Dosage compensation of sex-chromosome gene expression between male and female mammals is achieved via X chromosome inactivation (XCI) by employing epigenetic modifications to randomly silence one X chromosome during early embryogenesis. Human pluripotent stem cells (hPSCs) were reported to present various states of XCI that differ according to the expression of the long non-coding RNA XIST and the degree of X chromosome silencing. To obtain a comprehensive perspective on XCI in female hPSCs, we performed a large-scale analysis characterizing different XCI parameters in more than 700 RNA high-throughput sequencing samples. Our findings suggest differences in XCI status between most published samples of embryonic stem cells (ESCs) and induced PSCs (iPSCs). While the majority of iPSC lines maintain an inactive X chromosome, ESC lines tend to silence the expression of XIST and upregulate distal chromosomal regions. Our study highlights significant epigenetic heterogeneity within hPSCs, which may bear implications for their use in research and regenerative therapy.
Identifiants
pubmed: 30943402
pii: S2211-1247(19)30326-2
doi: 10.1016/j.celrep.2019.03.019
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
20-29.e3Informations de copyright
Copyright © 2019 The Author(s). Published by Elsevier Inc. All rights reserved.