exRNA Atlas Analysis Reveals Distinct Extracellular RNA Cargo Types and Their Carriers Present across Human Biofluids.


Journal

Cell
ISSN: 1097-4172
Titre abrégé: Cell
Pays: United States
ID NLM: 0413066

Informations de publication

Date de publication:
04 04 2019
Historique:
received: 28 04 2018
revised: 06 11 2018
accepted: 11 02 2019
entrez: 6 4 2019
pubmed: 6 4 2019
medline: 25 1 2020
Statut: ppublish

Résumé

To develop a map of cell-cell communication mediated by extracellular RNA (exRNA), the NIH Extracellular RNA Communication Consortium created the exRNA Atlas resource (https://exrna-atlas.org). The Atlas version 4P1 hosts 5,309 exRNA-seq and exRNA qPCR profiles from 19 studies and a suite of analysis and visualization tools. To analyze variation between profiles, we apply computational deconvolution. The analysis leads to a model with six exRNA cargo types (CT1, CT2, CT3A, CT3B, CT3C, CT4), each detectable in multiple biofluids (serum, plasma, CSF, saliva, urine). Five of the cargo types associate with known vesicular and non-vesicular (lipoprotein and ribonucleoprotein) exRNA carriers. To validate utility of this model, we re-analyze an exercise response study by deconvolution to identify physiologically relevant response pathways that were not detected previously. To enable wide application of this model, as part of the exRNA Atlas resource, we provide tools for deconvolution and analysis of user-provided case-control studies.

Identifiants

pubmed: 30951672
pii: S0092-8674(19)30167-9
doi: 10.1016/j.cell.2019.02.018
pmc: PMC6616370
mid: NIHMS1521746
pii:
doi:

Substances chimiques

Cell-Free Nucleic Acids 0
Circulating MicroRNA 0
RNA 63231-63-0

Types de publication

Dataset Journal Article Research Support, N.I.H., Extramural Research Support, N.I.H., Intramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

463-477.e15

Subventions

Organisme : NIA NIH HHS
ID : R01 AG059729
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL122547
Pays : United States
Organisme : NIDDK NIH HHS
ID : P30 DK063720
Pays : United States
Organisme : NCATS NIH HHS
ID : UH3 TR000906
Pays : United States
Organisme : NCI NIH HHS
ID : P50 CA217685
Pays : United States
Organisme : NCATS NIH HHS
ID : UH2 TR000906
Pays : United States
Organisme : NCI NIH HHS
ID : R35 CA209904
Pays : United States
Organisme : NCATS NIH HHS
ID : UH2 TR000923
Pays : United States
Organisme : NCATS NIH HHS
ID : UH3 TR000943
Pays : United States
Organisme : NIDA NIH HHS
ID : U54 DA036134
Pays : United States
Organisme : NCATS NIH HHS
ID : UH3 TR000891
Pays : United States
Organisme : NCATS NIH HHS
ID : UH3 TR000901
Pays : United States
Organisme : NCATS NIH HHS
ID : UH3 TR000923
Pays : United States
Organisme : NCATS NIH HHS
ID : UH2 TR000901
Pays : United States
Organisme : NHLBI NIH HHS
ID : K23 HL127099
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL133575
Pays : United States
Organisme : NIGMS NIH HHS
ID : R25 GM056929
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL136685
Pays : United States
Organisme : NCI NIH HHS
ID : U19 CA179512
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM008307
Pays : United States
Organisme : NHLBI NIH HHS
ID : U01 HL126494
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA163849
Pays : United States

Commentaires et corrections

Type : CommentIn

Informations de copyright

Copyright © 2019 Elsevier Inc. All rights reserved.

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Auteurs

Oscar D Murillo (OD)

Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA.

William Thistlethwaite (W)

Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA.

Joel Rozowsky (J)

Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520, USA.

Sai Lakshmi Subramanian (SL)

Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA.

Rocco Lucero (R)

Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA.

Neethu Shah (N)

Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA.

Andrew R Jackson (AR)

Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA.

Srimeenakshi Srinivasan (S)

Department of Obstetrics, Gynecology, and Reproductive Sciences and Sanford Consortium for Regenerative Medicine, University of California, San Diego, La Jolla, CA 92037, USA.

Allen Chung (A)

Department of Surgery, University of California, San Francisco, San Francisco, CA 94143, USA; Surgical Service, San Francisco Veterans Affairs Medical Center, San Francisco, CA 94121, USA.

Clara D Laurent (CD)

Department of Obstetrics, Gynecology, and Reproductive Sciences and Sanford Consortium for Regenerative Medicine, University of California, San Diego, La Jolla, CA 92037, USA.

Robert R Kitchen (RR)

Exosome Diagnostics, Inc., Waltham, MA 02451, USA.

Timur Galeev (T)

Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520, USA.

Jonathan Warrell (J)

Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520, USA.

James A Diao (JA)

Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520, USA; Harvard-MIT Division of Health Sciences and Technology, Harvard Medical School, Boston, MA 02115, USA.

Joshua A Welsh (JA)

Translational Nanobiology Section, Laboratory of Pathology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA.

Kristina Hanspers (K)

Gladstone Institutes, San Francisco, CA 94158, USA.

Anders Riutta (A)

Gladstone Institutes, San Francisco, CA 94158, USA.

Sebastian Burgstaller-Muehlbacher (S)

Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.

Ravi V Shah (RV)

Cardiovascular Research Center, Cardiology Division, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA.

Ashish Yeri (A)

Cardiovascular Research Center, Cardiology Division, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA.

Lisa M Jenkins (LM)

Laboratory of Cell Biology, Center for Cancer Research, NIH, Bethesda, MD 20892, USA.

Mehmet E Ahsen (ME)

Department of Genetics and Genomics Sciences, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA.

Carlos Cordon-Cardo (C)

Department of Pathology, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA.

Navneet Dogra (N)

Department of Genetics and Genomics Sciences, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA; IBM T.J. Watson Research Center, IBM Research, Yorktown Heights, NY 10598, USA.

Stacey M Gifford (SM)

IBM T.J. Watson Research Center, IBM Research, Yorktown Heights, NY 10598, USA.

Joshua T Smith (JT)

IBM T.J. Watson Research Center, IBM Research, Yorktown Heights, NY 10598, USA.

Gustavo Stolovitzky (G)

Department of Genetics and Genomics Sciences, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA; IBM T.J. Watson Research Center, IBM Research, Yorktown Heights, NY 10598, USA.

Ashutosh K Tewari (AK)

Department of Urology, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA.

Benjamin H Wunsch (BH)

IBM T.J. Watson Research Center, IBM Research, Yorktown Heights, NY 10598, USA.

Kamlesh K Yadav (KK)

Department of Urology, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA; Sema4, Stamford, CT 06902, USA.

Kirsty M Danielson (KM)

Cardiovascular Research Center, Cardiology Division, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA.

Justyna Filant (J)

Department of Gynecologic Oncology and Reproductive Medicine, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA.

Courtney Moeller (C)

Department of Obstetrics, Gynecology, and Reproductive Sciences and Sanford Consortium for Regenerative Medicine, University of California, San Diego, La Jolla, CA 92037, USA.

Parham Nejad (P)

Department of Neurology, Center for Neurologic Diseases, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.

Anu Paul (A)

Department of Neurology, Center for Neurologic Diseases, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.

Bridget Simonson (B)

Cardiovascular Research Center, Cardiology Division, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA.

David K Wong (DK)

Department of Surgery, University of California, San Francisco, San Francisco, CA 94143, USA; Surgical Service, San Francisco Veterans Affairs Medical Center, San Francisco, CA 94121, USA.

Xuan Zhang (X)

Exosome Diagnostics, Inc., Waltham, MA 02451, USA.

Leonora Balaj (L)

Department of Neurosurgery, Massachusetts General Hospital, Boston, MA 02114, USA.

Roopali Gandhi (R)

Department of Neurology, Center for Neurologic Diseases, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.

Anil K Sood (AK)

Department of Gynecologic Oncology and Reproductive Medicine, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA; Center for RNA Interference and Non-Coding RNAs, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA; Department of Cancer Biology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA.

Roger P Alexander (RP)

Pacific Northwest Research Institute, Seattle, WA 98122, USA.

Liang Wang (L)

Department of Pathology and MCW Cancer Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA.

Chunlei Wu (C)

Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.

David T W Wong (DTW)

School of Dentistry, University of California, Los Angeles, Los Angeles, CA 90095, USA.

David J Galas (DJ)

Pacific Northwest Research Institute, Seattle, WA 98122, USA.

Kendall Van Keuren-Jensen (K)

Neurogenomics, The Translational Genomics Research Institute (TGen), Phoenix, AZ 85004, USA.

Tushar Patel (T)

Department of Transplantation, Mayo Clinic, Jacksonville, FL 32224, USA.

Jennifer C Jones (JC)

Translational Nanobiology Section, Laboratory of Pathology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA.

Saumya Das (S)

Cardiovascular Research Center, Cardiology Division, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA.

Kei-Hoi Cheung (KH)

Department of Emergency Medicine, Yale University School of Medicine, New Haven, CT 06520, USA.

Alexander R Pico (AR)

Gladstone Institutes, San Francisco, CA 94158, USA.

Andrew I Su (AI)

Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.

Robert L Raffai (RL)

Department of Surgery, University of California, San Francisco, San Francisco, CA 94143, USA; Surgical Service, San Francisco Veterans Affairs Medical Center, San Francisco, CA 94121, USA.

Louise C Laurent (LC)

Department of Obstetrics, Gynecology, and Reproductive Sciences and Sanford Consortium for Regenerative Medicine, University of California, San Diego, La Jolla, CA 92037, USA.

Matthew E Roth (ME)

Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA.

Mark B Gerstein (MB)

Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520, USA; Program in Computational Biology & Bioinformatics, Yale University, New Haven, CT 06520, USA; Department of Computer Science, Yale University, New Haven, CT 06520, USA.

Aleksandar Milosavljevic (A)

Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA. Electronic address: amilosav@bcm.edu.

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Classifications MeSH