Enzyme promiscuity shapes adaptation to novel growth substrates.


Journal

Molecular systems biology
ISSN: 1744-4292
Titre abrégé: Mol Syst Biol
Pays: England
ID NLM: 101235389

Informations de publication

Date de publication:
08 04 2019
Historique:
entrez: 10 4 2019
pubmed: 10 4 2019
medline: 1 5 2020
Statut: epublish

Résumé

Evidence suggests that novel enzyme functions evolved from low-level promiscuous activities in ancestral enzymes. Yet, the evolutionary dynamics and physiological mechanisms of how such side activities contribute to systems-level adaptations are not well characterized. Furthermore, it remains untested whether knowledge of an organism's promiscuous reaction set, or underground metabolism, can aid in forecasting the genetic basis of metabolic adaptations. Here, we employ a computational model of underground metabolism and laboratory evolution experiments to examine the role of enzyme promiscuity in the acquisition and optimization of growth on predicted non-native substrates in

Identifiants

pubmed: 30962359
doi: 10.15252/msb.20188462
pmc: PMC6452873
doi:

Substances chimiques

Enzymes 0
Escherichia coli Proteins 0
Succinates 0
Tartrates 0
Deoxyribose 533-67-5
Arabinose B40ROO395Z
tartaric acid W4888I119H
monomethyl succinate YA2V724S0A

Types de publication

Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Langues

eng

Sous-ensembles de citation

IM

Pagination

e8462

Subventions

Organisme : Wellcome Trust
Pays : United Kingdom

Informations de copyright

© 2019 The Authors. Published under the terms of the CC BY 4.0 license.

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Auteurs

Gabriela I Guzmán (GI)

Department of Bioengineering, University of California, San Diego, La Jolla, CA, USA.

Troy E Sandberg (TE)

Department of Bioengineering, University of California, San Diego, La Jolla, CA, USA.

Ryan A LaCroix (RA)

Department of Bioengineering, University of California, San Diego, La Jolla, CA, USA.

Ákos Nyerges (Á)

Synthetic and Systems Biology Unit, Institute of Biochemistry, Biological Research Centre of the Hungarian Academy of Sciences, Szeged, Hungary.

Henrietta Papp (H)

Virological Research Group, Szentágothai Research Centre University of Pécs, Pécs, Hungary.

Markus de Raad (M)

Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory Berkeley, Berkeley, CA, USA.

Zachary A King (ZA)

Department of Bioengineering, University of California, San Diego, La Jolla, CA, USA.

Ying Hefner (Y)

Department of Bioengineering, University of California, San Diego, La Jolla, CA, USA.

Trent R Northen (TR)

Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory Berkeley, Berkeley, CA, USA.

Richard A Notebaart (RA)

Laboratory of Food Microbiology, Wageningen University and Research, Wageningen, The Netherlands.

Csaba Pál (C)

Synthetic and Systems Biology Unit, Institute of Biochemistry, Biological Research Centre of the Hungarian Academy of Sciences, Szeged, Hungary.

Bernhard O Palsson (BO)

Department of Bioengineering, University of California, San Diego, La Jolla, CA, USA.
Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Lyngby, Denmark.
Department of Pediatrics, University of California, San Diego, La Jolla, CA, USA.

Balázs Papp (B)

Synthetic and Systems Biology Unit, Institute of Biochemistry, Biological Research Centre of the Hungarian Academy of Sciences, Szeged, Hungary.

Adam M Feist (AM)

Department of Bioengineering, University of California, San Diego, La Jolla, CA, USA afeist@ucsd.edu.
Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Lyngby, Denmark.

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Classifications MeSH