Signal peptide peptidase activity connects the unfolded protein response to plant defense suppression by Ustilago maydis.
Aspartic Acid Endopeptidases
/ genetics
Endoplasmic Reticulum
/ metabolism
Endoplasmic Reticulum Stress
Fungal Proteins
/ genetics
Host-Pathogen Interactions
/ immunology
Plant Diseases
/ genetics
Protein Stability
Unfolded Protein Response
/ physiology
Ustilago
/ immunology
Virulence Factors
/ genetics
Zea mays
/ genetics
Journal
PLoS pathogens
ISSN: 1553-7374
Titre abrégé: PLoS Pathog
Pays: United States
ID NLM: 101238921
Informations de publication
Date de publication:
04 2019
04 2019
Historique:
received:
03
12
2018
accepted:
27
03
2019
revised:
30
04
2019
pubmed:
19
4
2019
medline:
19
10
2019
entrez:
19
4
2019
Statut:
epublish
Résumé
The corn smut fungus Ustilago maydis requires the unfolded protein response (UPR) to maintain homeostasis of the endoplasmic reticulum (ER) during the biotrophic interaction with its host plant Zea mays (maize). Crosstalk between the UPR and pathways controlling pathogenic development is mediated by protein-protein interactions between the UPR regulator Cib1 and the developmental regulator Clp1. Cib1/Clp1 complex formation results in mutual modification of the connected regulatory networks thereby aligning fungal proliferation in planta, efficient effector secretion with increased ER stress tolerance and long-term UPR activation in planta. Here we address UPR-dependent gene expression and its modulation by Clp1 using combinatorial RNAseq/ChIPseq analyses. We show that increased ER stress resistance is connected to Clp1-dependent alterations of Cib1 phosphorylation, protein stability and UPR gene expression. Importantly, we identify by deletion screening of UPR core genes the signal peptide peptidase Spp1 as a novel key factor that is required for establishing a compatible biotrophic interaction between U. maydis and its host plant maize. Spp1 is dispensable for ER stress resistance and vegetative growth but requires catalytic activity to interfere with the plant defense, revealing a novel virulence specific function for signal peptide peptidases in a biotrophic fungal/plant interaction.
Identifiants
pubmed: 30998787
doi: 10.1371/journal.ppat.1007734
pii: PPATHOGENS-D-18-02303
pmc: PMC6490947
doi:
Substances chimiques
Fungal Proteins
0
Virulence Factors
0
Aspartic Acid Endopeptidases
EC 3.4.23.-
signal peptide peptidase
EC 3.4.23.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e1007734Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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