Precise editing of plant genomes - Prospects and challenges.
CRISPR/Cas
Genome editing
Plants
Safety regulation
Journal
Seminars in cell & developmental biology
ISSN: 1096-3634
Titre abrégé: Semin Cell Dev Biol
Pays: England
ID NLM: 9607332
Informations de publication
Date de publication:
12 2019
12 2019
Historique:
received:
31
12
2018
revised:
15
04
2019
accepted:
15
04
2019
pubmed:
20
4
2019
medline:
6
5
2020
entrez:
20
4
2019
Statut:
ppublish
Résumé
The past decade has witnessed unprecedented development in genome engineering, a process that enables targeted modification of genomes. The identification of sequence-specific nucleases such as zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs) and the CRISPR/Cas system, in particular, has led to precise and efficient introduction of genetic variations into genomes of various organisms. Since the CRISPR/Cas system is highly versatile, cost-effective and much superior to ZFNs and TALENs, its widespread adoption by the research community has been inevitable. In plants, a number of studies have shown that CRISPR/Cas could be a potential tool in basic research where insertion, deletion and/or substitution in the genetic sequence could help answer fundamental questions about plant processes, and in applied research these technologies could help build or reverse-engineer plant systems to make them more useful. In this review article, we summarize technologies for precise editing of genomes with a special focus on the CRISPR/Cas system, highlight the latest developments in the CRISPR/Cas system and discuss the challenges and prospects in using the system for plant biology research.
Identifiants
pubmed: 31002868
pii: S1084-9521(18)30106-X
doi: 10.1016/j.semcdb.2019.04.010
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
115-123Informations de copyright
Copyright © 2019 Elsevier Ltd. All rights reserved.