LC-MS/MS assay for the simultaneous determination of tocopherols, polyunsaturated fatty acids and their metabolites in human plasma and serum.
Eicosanoids
Human plasma/serum
Liquid-chromatography tandem-mass spectrometry (LC-MS/MS)
Polyunsaturated fatty acids
Tocopheryl quinone
Validation
Vitamin E
Journal
Free radical biology & medicine
ISSN: 1873-4596
Titre abrégé: Free Radic Biol Med
Pays: United States
ID NLM: 8709159
Informations de publication
Date de publication:
20 11 2019
20 11 2019
Historique:
received:
26
02
2019
revised:
05
04
2019
accepted:
15
04
2019
pubmed:
23
4
2019
medline:
8
9
2020
entrez:
23
4
2019
Statut:
ppublish
Résumé
The role of vitamin E in both enzymatic and free radical-dependent metabolism of polyunsaturated fatty acids (PUFAs) has been well demonstrated. This study proposed a new LC-MS/MS method to quantify the main vitamin E forms, their metabolites and main PUFA species in human blood, since, at present, there are not procedures able to simultaneously determine these two classes of compounds. After the optimization of sample treatment and reverse-phase separation conditions, tandem mass spectrometry detection was evaluated experimenting both positive and negative electrospray ionisation modes. The procedure was also preliminarily adapted to assess five arachidonic acid-derived eicosanoids that could be under the influence of vitamin E function, such as LTB4 (leukotriene B4), 20-HETE (20-hydroxyeicosatetraenoic acid) and their ω-oxidation metabolites. After the validation study, the performance characteristics were confirmed analysing a certified reference material (SRM
Identifiants
pubmed: 31009660
pii: S0891-5849(18)32425-0
doi: 10.1016/j.freeradbiomed.2019.04.017
pii:
doi:
Substances chimiques
Eicosanoids
0
Fatty Acids, Unsaturated
0
Hydroxyeicosatetraenoic Acids
0
Vitamin E
1406-18-4
Leukotriene B4
1HGW4DR56D
Arachidonic Acid
27YG812J1I
20-hydroxy-5,8,11,14-eicosatetraenoic acid
79551-86-3
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Validation Study
Langues
eng
Sous-ensembles de citation
IM
Pagination
134-143Informations de copyright
Copyright © 2019 Elsevier Inc. All rights reserved.