Light-dependent N-terminal phosphorylation of LHCSR3 and LHCB4 are interlinked in Chlamydomonas reinhardtii.
high light stress
light harvesting
photosynthesis
protein phosphorylation
Journal
The Plant journal : for cell and molecular biology
ISSN: 1365-313X
Titre abrégé: Plant J
Pays: England
ID NLM: 9207397
Informations de publication
Date de publication:
09 2019
09 2019
Historique:
received:
31
07
2018
revised:
15
03
2019
accepted:
16
04
2019
pubmed:
30
4
2019
medline:
1
7
2020
entrez:
30
4
2019
Statut:
ppublish
Résumé
Phosphorylation dynamics of LHCSR3 were investigated in Chlamydomonas reinhardtii by quantitative proteomics and genetic engineering. LHCSR3 protein expression and phosphorylation were induced in high light. Our data revealed synergistic and dynamic N-terminal LHCSR3 phosphorylation. Phosphorylated and nonphosphorylated LHCSR3 associated with PSII-LHCII supercomplexes. The phosphorylation status of LHCB4 was closely linked to the phosphorylation of multiple sites at the N-terminus of LHCSR3, indicating that LHCSR3 phosphorylation may operate as a molecular switch modulating LHCB4 phosphorylation, which in turn is important for PSII-LHCII disassembly. Notably, LHCSR3 phosphorylation diminished under prolonged high light, which coincided with onset of CEF. Hierarchical clustering of significantly altered proteins revealed similar expression profiles of LHCSR3, CRX, and FNR. This finding indicated the existence of a functional link between LHCSR3 protein abundance and phosphorylation, photosynthetic electron flow, and the oxidative stress response.
Identifiants
pubmed: 31033075
doi: 10.1111/tpj.14368
pmc: PMC6851877
doi:
Substances chimiques
Light-Harvesting Protein Complexes
0
Photosystem II Protein Complex
0
Plant Proteins
0
Protein Kinases
EC 2.7.-
light-harvesting complex II kinase
EC 2.7.1.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
877-894Informations de copyright
© 2019 The Authors The Plant Journal published by Society for Experimental Biology and John Wiley & Sons Ltd.
Références
Plant Cell. 2011 Aug;23(8):2950-63
pubmed: 21856795
Nucleic Acids Res. 2016 Jan 4;44(D1):D447-56
pubmed: 26527722
Plant Physiol. 2015 Apr;167(4):1566-78
pubmed: 25699588
Photosynth Res. 2017 Dec;134(3):291-306
pubmed: 28593495
Photosynth Res. 2013 Oct;116(2-3):251-63
pubmed: 23595278
PLoS One. 2012;7(8):e43349
pubmed: 22937037
Nature. 2009 Nov 26;462(7272):518-21
pubmed: 19940928
Science. 2003 Mar 7;299(5612):1572-5
pubmed: 12624266
J Biol Chem. 2005 May 27;280(21):20431-41
pubmed: 15774469
Proc Natl Acad Sci U S A. 2010 Mar 9;107(10):4782-7
pubmed: 20176943
J Am Chem Soc. 2013 Dec 11;135(49):18339-42
pubmed: 24261574
Nature. 1970 Aug 15;227(5259):680-5
pubmed: 5432063
Int J Phytoremediation. 2017 Dec 2;19(12):1085-1092
pubmed: 28541720
FEBS Lett. 2004 Apr 23;564(1-2):104-8
pubmed: 15094049
Proc Natl Acad Sci U S A. 2018 Oct 9;115(41):10517-10522
pubmed: 30254175
Proc Natl Acad Sci U S A. 2009 Jun 16;106(24):9908-13
pubmed: 19470473
Proc Natl Acad Sci U S A. 2013 Jun 11;110(24):10016-21
pubmed: 23716695
Annu Rev Genet. 2008;42:463-515
pubmed: 18983262
J Biol Chem. 2014 Jul 18;289(29):20150-7
pubmed: 24914208
J Biol Chem. 2012 Sep 7;287(37):31574-81
pubmed: 22801422
Plant Cell Physiol. 2014 Aug;55(8):1437-49
pubmed: 24867888
Plant Cell. 2013 Feb;25(2):545-57
pubmed: 23424243
Cell. 2017 Sep 21;171(1):133-147.e14
pubmed: 28938113
J Biol Chem. 1991 May 5;266(13):8136-42
pubmed: 2022634
Proc Natl Acad Sci U S A. 2011 Aug 9;108(32):13317-22
pubmed: 21784980
Nat Biotechnol. 2008 Dec;26(12):1367-72
pubmed: 19029910
Proteomics. 2007 Nov;7(21):3964-79
pubmed: 17922516
J Proteome Res. 2014 Sep 5;13(9):4205-10
pubmed: 25102069
Nature. 1961 Jul 8;191:144-8
pubmed: 13771349
Plant Physiol. 2015 Jun;168(2):615-34
pubmed: 25858915
Biochim Biophys Acta Bioenerg. 2017 May;1858(5):379-385
pubmed: 28257778
Mol Cell Proteomics. 2008 Apr;7(4):661-71
pubmed: 18039691
Plant Cell Physiol. 2014 Jul;55(7):1304-10
pubmed: 24850838
Plant Cell. 2014 Jul;26(7):3036-50
pubmed: 24989042
Proc Natl Acad Sci U S A. 2016 Dec 20;113(51):14864-14869
pubmed: 27930292
PLoS One. 2011;6(9):e24565
pubmed: 21915352
Proc Natl Acad Sci U S A. 2002 Jul 23;99(15):10209-14
pubmed: 12119384
Plant Physiol. 2014 May;165(1):438-52
pubmed: 24623849
J Biol Chem. 2009 Mar 20;284(12):7777-82
pubmed: 19144643
Plant Physiol. 2014 Jun 19;165(4):1604-1617
pubmed: 24948831
Plant Cell. 2008 Aug;20(8):2177-89
pubmed: 18757554
J Biol Chem. 2016 Aug 12;291(33):17478-87
pubmed: 27358399
Proc Natl Acad Sci U S A. 2006 Jan 10;103(2):477-82
pubmed: 16407170
Proc Natl Acad Sci U S A. 1963 Feb 15;49:266-70
pubmed: 14000214
J Biol Chem. 2016 Apr 1;291(14):7334-46
pubmed: 26817847
Mol Cell Proteomics. 2013 Oct;12(10):2774-90
pubmed: 23820728
J Biol Chem. 2016 Oct 7;291(41):21740-21750
pubmed: 27539852
Nature. 2000 Jan 27;403(6768):391-5
pubmed: 10667783
Photosynth Res. 2013 Nov;117(1-3):449-59
pubmed: 24113925
Nature. 2010 Apr 22;464(7292):1210-3
pubmed: 20364124
Proc Natl Acad Sci U S A. 2014 Mar 4;111(9):3460-5
pubmed: 24550508
Plant Cell. 2016 Apr;28(4):966-83
pubmed: 27020958
Proc Natl Acad Sci U S A. 2016 Mar 15;113(11):E1516-25
pubmed: 26929361
Mol Cell Proteomics. 2010 Jun;9(6):1281-95
pubmed: 20124224
Mol Plant. 2014 Jul;7(7):1252-5
pubmed: 24658415
Proc Natl Acad Sci U S A. 2014 Apr 1;111(13):5042-7
pubmed: 24639515
Biochim Biophys Acta. 2016 Jan;1857(1):1-6
pubmed: 26235611
Photosynth Res. 2010 Nov;106(1-2):179-89
pubmed: 20632109
Mol Gen Genet. 1996 Apr 24;251(1):23-30
pubmed: 8628243
Bioinformatics. 2010 Apr 1;26(7):966-8
pubmed: 20147306
Proc Natl Acad Sci U S A. 1997 Feb 18;94(4):1585-90
pubmed: 11038603
PLoS Biol. 2010 Jan 26;8(1):e1000288
pubmed: 20126264
EMBO J. 1999 Jun 1;18(11):2961-9
pubmed: 10357809
Plant Physiol. 2016 Aug;171(4):2717-30
pubmed: 27329221
Biochim Biophys Acta. 2005 Jan 7;1706(1-2):12-39
pubmed: 15620363
J Biol Chem. 1950 Aug;185(2):781-7
pubmed: 14774424
Proc Natl Acad Sci U S A. 1975 Jun;72(6):2175-9
pubmed: 1056023
Nat Methods. 2009 May;6(5):359-62
pubmed: 19377485
Plant Cell. 2015 Nov;27(11):3213-27
pubmed: 26508763
Plant J. 2016 Sep;87(5):484-94
pubmed: 27214592
Nature. 2004 Jun 3;429(6991):579-82
pubmed: 15175756
Proc Natl Acad Sci U S A. 2012 Oct 23;109(43):17717-22
pubmed: 23045639
Nature. 2005 Feb 24;433(7028):892-5
pubmed: 15729347
Nat Commun. 2016 Jun 14;7:11847
pubmed: 27297041
Nature. 1959 Jul 4;184:10-21
pubmed: 13794394
Nature. 2016 May 18;534(7605):69-74
pubmed: 27251276
Nat Methods. 2016 Sep;13(9):731-40
pubmed: 27348712
Mymensingh Med J. 2017 Apr;26(2):439-447
pubmed: 28588184
Nat Plants. 2016 Apr 04;2:16031
pubmed: 27249564
J Biol Chem. 2001 Oct 12;276(41):38159-65
pubmed: 11483610
PLoS Biol. 2011 Jan 18;9(1):e1000577
pubmed: 21267060
PLoS Biol. 2009 Mar 3;7(3):e45
pubmed: 19260761
Nature. 2016 Sep 22;537(7621):563-566
pubmed: 27626383