First evidence of cholinesterase-like activity in Basidiomycota.
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2019
2019
Historique:
received:
26
02
2019
accepted:
13
04
2019
entrez:
1
5
2019
pubmed:
1
5
2019
medline:
9
1
2020
Statut:
epublish
Résumé
Cholinesterases (ChE), the enzymes whose primary function is the hydrolysis of choline esters, are widely expressed throughout the nature. Although they have already been found in plants and microorganisms, including ascomycete fungi, this study is the first report of ChE-like activity in fungi of the phylum Basidiomycota. This activity was detected in almost a quarter of the 45 tested aqueous fungal extracts. The ability of these extracts to hydrolyse acetylthiocholine was about ten times stronger than the hydrolytic activity towards butyrylthiocholine and propionylthiocholine. In-gel detection of ChE-like activity with acetylthiocholine indicated a great variability in the characteristics of these enzymes which are not characterized as vertebrate-like based on (i) differences in inhibition by excess substrate, (ii) susceptibility to different vertebrate acetylcholinesterase and butyrylcholinesterase inhibitors, and (iii) a lack of orthologs using phylogenetic analysis. Limited inhibition by single inhibitors and multiple activity bands using in-gel detection indicate the presence of several ChE-like enzymes in these aqueous extracts. We also observed inhibitory activity of the same aqueous mushroom extracts against insect acetylcholinesterase in 10 of the 45 samples tested; activity was independent of the presence of ChE-like activity in extracts. Both ChE-like activities with different substrates and the ability of extracts to inhibit insect acetylcholinesterase were not restricted to any fungal family but were rather present across all included Basidiomycota families. This study can serve as a platform for further research regarding ChE activity in mushrooms.
Identifiants
pubmed: 31039204
doi: 10.1371/journal.pone.0216077
pii: PONE-D-19-05648
pmc: PMC6490906
doi:
Substances chimiques
Cholinesterase Inhibitors
0
Lipase
EC 3.1.1.3
Cholinesterases
EC 3.1.1.8
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0216077Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
Références
Mikrobiologiia. 1975 Jul-Aug;44(4):761-2
pubmed: 809648
Chem Biol Interact. 2008 Sep 25;175(1-3):3-10
pubmed: 18586019
J Gen Microbiol. 1953 Feb;8(1):8-17
pubmed: 13035026
Bull Environ Contam Toxicol. 2003 Aug;71(2):219-25
pubmed: 14560370
Bioorg Med Chem. 2010 Mar 15;18(6):2173-2177
pubmed: 20176490
Comp Biochem Physiol Part D Genomics Proteomics. 2012 Jun;7(2):83-93
pubmed: 22210164
Comp Biochem Physiol C Toxicol Pharmacol. 2002 May;132(1):85-92
pubmed: 12039688
Protein Pept Lett. 2012 Feb;19(2):165-72
pubmed: 21933122
Jpn J Pharmacol. 2001 Jan;85(1):2-10
pubmed: 11243568
PLoS One. 2011 Apr 27;6(4):e19063
pubmed: 21556353
Nucleic Acids Res. 2016 Jul 8;44(W1):W242-5
pubmed: 27095192
Sci Rep. 2017 Jan 03;7:39864
pubmed: 28045085
Annu Rev Neurosci. 1982;5:57-106
pubmed: 6176173
Prog Neurobiol. 1993 Jul;41(1):31-91
pubmed: 8321908
Microb Pathog. 1991 Aug;11(2):101-10
pubmed: 1961106
Pharmacol Ther. 2015 Apr;148:34-46
pubmed: 25448037
Biochim Biophys Acta. 1998 Jun 11;1385(1):126-38
pubmed: 9630567
J Histochem Cytochem. 1964 Mar;12:219-21
pubmed: 14187330
Chemosphere. 2006 Dec;65(10):1731-7
pubmed: 17228426
Mol Pharmacol. 1991 Jan;39(1):98-104
pubmed: 1987454
Mol Biol Evol. 2013 Apr;30(4):772-80
pubmed: 23329690
Life Sci. 2003 Feb 28;72(15):1745-56
pubmed: 12559395
Biochem Pharmacol. 2018 Jul;153:205-216
pubmed: 29409903
Insect Biochem Mol Biol. 2013 Jan;43(1):47-53
pubmed: 23168079
Chem Biol Interact. 2013 Mar 25;203(1):10-3
pubmed: 23085121
Appl Biochem Biotechnol. 2012 Nov;168(5):991-1002
pubmed: 22945561
Protein Pept Lett. 2012 Feb;19(2):132-43
pubmed: 21933125
Arch Pharm Res. 2013 Apr;36(4):375-99
pubmed: 23435942
J Biol Chem. 2003 Oct 17;278(42):41141-7
pubmed: 12869558
Neurochem Int. 1980;2C:161-84
pubmed: 20487781
Nat Prod Rep. 2006 Apr;23(2):181-99
pubmed: 16572227
Planta. 2008 Mar;227(4):809-22
pubmed: 18046576
World J Microbiol Biotechnol. 2016 Feb;32(2):20
pubmed: 26745980
Nucleic Acids Res. 2013 Jan;41(Database issue):D423-9
pubmed: 23193256
Chem Biol Interact. 2013 Mar 25;203(1):266-8
pubmed: 23010363
J Mol Neurosci. 2003;20(3):369-83
pubmed: 14501022
Proc Soc Exp Biol Med. 1951 Jan;76(1):169-70
pubmed: 14816428
Chem Biol Interact. 2010 Sep 6;187(1-3):27-33
pubmed: 20359467
Biochem Pharmacol. 2003 Feb 1;65(3):407-16
pubmed: 12527333
Science. 1991 Aug 23;253(5022):872-9
pubmed: 1678899
Mycobiology. 2016 Dec;44(4):291-301
pubmed: 28154487
J Exp Zool. 1999 Jan 1;283(1):102-5
pubmed: 9990739
Biochem Pharmacol. 1961 Jul;7:88-95
pubmed: 13726518
Biochem Biophys Res Commun. 2015 Sep 25;465(3):488-93
pubmed: 26277389
Nauchnye Doki Vyss Shkoly Biol Nauki. 1991;(12):50-4
pubmed: 1790247
Microbiol Res. 2012 Jun 20;167(6):317-25
pubmed: 22192836
Chem Biol Interact. 2010 Sep 6;187(1-3):34-43
pubmed: 20226178
Chemosphere. 2002 Aug;48(6):563-9
pubmed: 12143930
Mar Environ Res. 2004 Apr;57(3):155-69
pubmed: 14580806
Mol Neurobiol. 2015 Aug;52(1):45-56
pubmed: 25112677
FEMS Microbiol Lett. 1991 Aug 1;66(2):131-5
pubmed: 1657699
Sci Rep. 2012;2:288
pubmed: 22371826
Appl Microbiol Biotechnol. 2000 Aug;54(2):141-52
pubmed: 10968625
Pharm Biol. 2015 Jun;53(6):824-30
pubmed: 25720975
Biochem J. 1990 Jul 1;269(1):279-80
pubmed: 2115773
Arch Insect Biochem Physiol. 2007 Nov;66(3):122-34
pubmed: 17966129
PLoS One. 2010 Mar 10;5(3):e9490
pubmed: 20224823
Chemosphere. 2002 Jul;48(4):407-14
pubmed: 12152742
Nucleic Acids Res. 2016 Jan 4;44(D1):D279-85
pubmed: 26673716
Biochimie. 2011 Oct;93(10):1685-93
pubmed: 21672601
Molecules. 2013 Jun 07;18(6):6663-78
pubmed: 23749158
J Plant Physiol. 2011 Nov 1;168(16):1987-92
pubmed: 21757255
Microbiology. 1995 Jun;141 ( Pt 6):1309-14
pubmed: 7670634
PLoS One. 2012;7(11):e48838
pubmed: 23144990
Neuroscience. 2013 Mar 27;234:53-68
pubmed: 23305761
Toxicology. 2007 Apr 20;233(1-3):60-9
pubmed: 17194517
Appl Environ Microbiol. 2009 Feb;75(3):792-801
pubmed: 19047386
Br J Pharmacol. 2008 Aug;154(8):1558-71
pubmed: 18500366
Eur J Biochem. 2003 Jan;270(2):315-24
pubmed: 12605682
Nature. 1991 Jun 27;351(6329):761-4
pubmed: 2062369
Plant Physiol. 2005 Jul;138(3):1359-71
pubmed: 15980188
J Neurochem. 1987 Nov;49(5):1455-61
pubmed: 3668532
Curr Opin Pharmacol. 2005 Jun;5(3):293-302
pubmed: 15907917
Prog Neurobiol. 1989;32(5):423-46
pubmed: 2660188
Biochem J. 1914 Feb;8(1):44-9
pubmed: 16742288