An Interscholastic Network To Generate LexA Enhancer Trap Lines in

Animals Animals, Genetically Modified Bacterial Proteins / genetics Base Sequence Binding Sites Drosophila / genetics Drosophila melanogaster / genetics Enhancer Elements, Genetic Female Gene Expression Regulation Genes, Reporter Genetic Loci Homologous Recombination Male Organ Specificity Position-Specific Scoring Matrices Protein Binding Serine Endopeptidases / genetics

Drosophila melanogaster Enhancer trap High School - University genetics course collaboration LexA - LexAop binary expression system STEM StanEx

Journal

G3 (Bethesda, Md.)

ISSN: 2160-1836

Titre abrégé: G3 (Bethesda)

Pays: England

ID NLM: 101566598

Informations de publication

Date de publication:
09 07 2019

Historique:

pubmed: 2 5 2019

medline: 16 1 2020

entrez: 2 5 2019

Statut: epublish

Résumé

Binary expression systems like the LexA-LexAop system provide a powerful experimental tool kit to study gene and tissue function in developmental biology, neurobiology, and physiology. However, the number of well-defined LexA enhancer trap insertions remains limited. In this study, we present the molecular characterization and initial tissue expression analysis of nearly 100 novel StanEx LexA enhancer traps, derived from the

Identifiants

DOI: 10.1534/g3.119.400105 PMID: 31040111 PMC: PMC6643891

pubmed: 31040111

pii: g3.119.400105

doi: 10.1534/g3.119.400105

pmc: PMC6643891

doi:

Substances chimiques

Bacterial Proteins 0

LexA protein, Bacteria 0

Serine Endopeptidases EC 3.4.21.-

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

Pagination

2097-2106

Subventions

Organisme : NIDDK NIH HHS

ID : P30 DK116074

Pays : United States

Informations de copyright

Références

Genetica. 2007 Sep;131(1):21-8

pubmed: 17318316

Science. 1910 Jul 22;32(812):120-2

pubmed: 17759620

Proc Natl Acad Sci U S A. 1995 Nov 21;92(24):10824-30

pubmed: 7479892

G3 (Bethesda). 2016 Oct 13;6(10):3017-3026

pubmed: 27527793

Annu Rev Genet. 1990;24:543-78

pubmed: 1965103

Genesis. 2002 Sep-Oct;34(1-2):58-61

pubmed: 12324948

Nucleic Acids Res. 2008 Nov;36(19):6199-208

pubmed: 18829720

Cell. 2013 Nov 21;155(5):1141-53

pubmed: 24267893

Genetics. 1994 Dec;138(4):1127-35

pubmed: 7896095

Genome. 2011 Sep;54(9):752-62

pubmed: 21888571

Proc Natl Acad Sci U S A. 1989 Dec;86(23):9402-6

pubmed: 2556711

Cell. 1983 Aug;34(1):25-35

pubmed: 6309410

Proc Natl Acad Sci U S A. 2000 May 9;97(10):5351-6

pubmed: 10805795

Methods Mol Biol. 2014;1196:49-57

pubmed: 25151157

Nat Neurosci. 2006 May;9(5):703-9

pubmed: 16582903

Mol Cell Biol. 1988 Apr;8(4):1489-97

pubmed: 2837644

Genetics. 2010 Oct;186(2):735-55

pubmed: 20697123

EMBO J. 1987 Dec 20;6(13):4125-35

pubmed: 2832152

Genetics. 2015 Apr;199(4):919-34

pubmed: 25680812

Neuron. 2009 Feb 12;61(3):373-84

pubmed: 19217375

Nucleic Acids Res. 2015 Apr 30;43(8):e56

pubmed: 25712101

Genes Genet Syst. 2008 Feb;83(1):67-75

pubmed: 18379135

Genetics. 2011 Jul;188(3):731-43

pubmed: 21515576

Elife. 2018 Aug 17;7:

pubmed: 30117808

Microbiol Spectr. 2015 Apr;3(2):MDNA3-0004-2014

pubmed: 26104714

Development. 2015 Feb 1;142(3):597-606

pubmed: 25605786

Trends Genet. 1991 Sep;7(9):282-7

pubmed: 1662417

Nat Genet. 2004 Mar;36(3):283-7

pubmed: 14981521

Mech Dev. 2006 Nov;123(11):860-7

pubmed: 16971094

J Neurogenet. 2016 Sep - Dec;30(3-4):185-194

pubmed: 27846759

Nat Commun. 2015 Dec 04;6:10024

pubmed: 26635273

Genome Res. 2004 Jun;14(6):1188-90

pubmed: 15173120

Proc Natl Acad Sci U S A. 2010 Sep 14;107(37):16166-71

pubmed: 20805468

Genes Dev. 1995 May 1;9(9):1137-48

pubmed: 7744254

Genome Res. 2015 Mar;25(3):445-58

pubmed: 25589440

Genetics. 2016 Aug;203(4):1513-31

pubmed: 27516614

PLoS Genet. 2006 Jun;2(6):e92

pubmed: 16789831

Proc Natl Acad Sci U S A. 1999 Jun 8;96(12):6856-61

pubmed: 10359803

Genetics. 1988 Mar;118(3):461-70

pubmed: 2835286

Genetics. 2017 Jun;206(2):775-784

pubmed: 28363977

Development. 1993 Jun;118(2):401-15

pubmed: 8223268

J Neurogenet. 2017 Dec;31(4):288-299

pubmed: 29119859

Nat Commun. 2019 Oct 22;10(1):4796

pubmed: 31641138

Nat Methods. 2011 Mar;8(3):231-7

pubmed: 21473015

G3 (Bethesda). 2016 Jul 07;6(7):1891-7

pubmed: 27172198

Genetics. 1991 Mar;127(3):515-24

pubmed: 1849859

PLoS Biol. 2012;10(7):e1001356

pubmed: 22802724

Proc Natl Acad Sci U S A. 1987 Dec;84(24):9123-7

pubmed: 2827169

Dev Cell. 2011 Jul 19;21(1):29-31

pubmed: 21763605