A new role for RU486 (mifepristone): it protects sperm from premature capacitation during cryopreservation in buffalo.
Animals
Antioxidants
/ pharmacology
Buffaloes
Calcium
/ metabolism
Calcium Channels
/ metabolism
Calcium Signaling
Cell Membrane
/ chemistry
Cholesterol
/ metabolism
Cryopreservation
/ methods
Egg Yolk
/ chemistry
Female
Fertilization in Vitro
Hormone Antagonists
/ pharmacology
Male
Mifepristone
/ pharmacology
Progesterone
/ metabolism
Semen
/ metabolism
Semen Preservation
/ methods
Sperm Capacitation
/ drug effects
Sperm Motility
Spermatozoa
/ cytology
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
30 04 2019
30 04 2019
Historique:
received:
11
05
2018
accepted:
15
04
2019
entrez:
2
5
2019
pubmed:
2
5
2019
medline:
15
12
2020
Statut:
epublish
Résumé
The objective of this study was to determine the mechanism by which RU 486 (mifepristone) protects sperm to undergo premature capacitation during cryopreservation. For this, semen ejaculate (n = 20) was divided into four equal fractions and diluted using egg yolk-based extender supplemented with different concentrations of RU 486 (0, 5, 10 and 20 µM) and cryopreserved. We found that RU 486 did not impair the post-thaw sperm kinetics and motility but prevented cholesterol efflux, calcium influx, and protected CatSper channels during cryopreservation. The RU 486 protected sperm from premature capacitation which was confirmed by intracellular calcium level, expression of tyrosine phosphorylated proteins (75 and 80 kDa) and CTC (chlortetracycline) assay. Furthermore, antioxidant ability of RU 486 was reflected by the ferric reducing ability, lower production of sperm malondialdehyde and intracellular reactive oxygen species. Also, we demonstrated that RU 486 treated sperm underwent normal capacitation, zona pellucida binding and zygote cleavage indicating normal fertilizing ability of sperm. In conclusion, we report a new role of RU 486 in protecting buffalo sperm from premature capacitation during cryopreservation.
Identifiants
pubmed: 31040304
doi: 10.1038/s41598-019-43038-4
pii: 10.1038/s41598-019-43038-4
pmc: PMC6491824
doi:
Substances chimiques
Antioxidants
0
Calcium Channels
0
Hormone Antagonists
0
Mifepristone
320T6RNW1F
Progesterone
4G7DS2Q64Y
Cholesterol
97C5T2UQ7J
Calcium
SY7Q814VUP
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
6712Commentaires et corrections
Type : ErratumIn
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