Paracrine/autocrine control of spermatogenesis by gonadotropin-inhibitory hormone.
Animals
Autocrine Communication
/ drug effects
Gene Expression Regulation, Developmental
/ drug effects
Gonadotropins
/ pharmacology
Leydig Cells
/ metabolism
Male
Neuropeptides
/ genetics
Paracrine Communication
/ drug effects
Spermatids
/ growth & development
Spermatogenesis
/ drug effects
Zebrafish
/ growth & development
Zebrafish Proteins
/ genetics
Flutamide (FLU)
Gonadotropin-inhibitory hormone (Gnih)
Human chorionic gonadotropin (hCG)
Spermatids (SPD)
Spermatozoa (SPZ)
Testosterone
Zebrafish gnih (zGnih)
Journal
Molecular and cellular endocrinology
ISSN: 1872-8057
Titre abrégé: Mol Cell Endocrinol
Pays: Ireland
ID NLM: 7500844
Informations de publication
Date de publication:
15 07 2019
15 07 2019
Historique:
received:
06
03
2019
revised:
25
04
2019
accepted:
26
04
2019
pubmed:
3
5
2019
medline:
9
6
2020
entrez:
4
5
2019
Statut:
ppublish
Résumé
Control of testicular development is multifactorial and involves a number of hypothalamic, hypophyseal and peripheral hormones. Here, we investigated direct action of zebrafish gonadotropin-inhibitory hormone (zGnih) which is expressed in the testis, on spermatogenesis in zebrafish, in vitro. Treatment with zGnih at the lower doses (10 and 100 nM) inhibited gonadotropin-induced spermatids/spermatozoa (SPD/SPZ) production. However, at the highest dose (1000 nM), zGnih increased basal number of SPD/SPZ and showed paradoxical effect. The effects of zGnih on testosterone and SPD/SPZ production was blocked in the presence of androgen receptor antagonist, flutamide (FLU). A number of transcripts were also measured to better understand zGnih mechanisms of action on zebrafish spermatogenesis. Our results provide strong support for the hypothesis that locally produced zGnih is a component of the complex multifactorial system that regulates testicular development and function in adult zebrafish, in part, by changes in testicular steroidogenesis and regulation of gonadotropin-induced response.
Identifiants
pubmed: 31048005
pii: S0303-7207(19)30134-0
doi: 10.1016/j.mce.2019.04.020
pii:
doi:
Substances chimiques
Gonadotropins
0
Neuropeptides
0
Zebrafish Proteins
0
neuropeptide VF
0
npvf protein, zebrafish
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
110440Informations de copyright
Copyright © 2019. Published by Elsevier B.V.