Phenylketonuria Diet Promotes Shifts in Firmicutes Populations.
Cluster Analysis
DNA, Bacterial
/ chemistry
DNA, Ribosomal
/ chemistry
Diet
/ methods
Diet Therapy
/ methods
Fatty Acids
/ analysis
Feces
/ chemistry
Firmicutes
/ drug effects
Gastrointestinal Microbiome
/ drug effects
Humans
Phenylketonurias
/ therapy
Phylogeny
RNA, Ribosomal, 16S
/ genetics
Sequence Analysis, DNA
Faecalibacterium prausnitzii
butyrate
diet
glycemic index
microbiota
mild hyperphenylalaninemia
phenylketonuria
Journal
Frontiers in cellular and infection microbiology
ISSN: 2235-2988
Titre abrégé: Front Cell Infect Microbiol
Pays: Switzerland
ID NLM: 101585359
Informations de publication
Date de publication:
2019
2019
Historique:
received:
08
02
2019
accepted:
25
03
2019
entrez:
7
5
2019
pubmed:
7
5
2019
medline:
18
12
2019
Statut:
epublish
Résumé
Low-phenylalanine diet, the mainstay of treatment for phenylketonuria (PKU), has been shown to increase glycemic index and glycemic load, affecting the availability of substrates for microbial fermentation. Indeed, changes in the PKU gut microbiota compared with healthy controls have been previously reported. In this study we compared the gut microbial communities of children with PKU and with mild hyperphenylalaninemia (MHP, unrestricted diet). For each group, we enrolled 21 children (4-18 years old), for a total dataset of 42 subjects. We assessed dietary intake and performed gut microbiota analysis by sequencing the V3-V4 hypervariable regions of the 16S rRNA gene. Short chain fatty acids (SCFAs) were quantified by gas chromatographic analysis. While alpha-diversity analysis showed no significant differences between PKU and MHP groups, microbial community analysis highlighted a significant separation of the gut microbiota according to both unweighted (
Identifiants
pubmed: 31058098
doi: 10.3389/fcimb.2019.00101
pmc: PMC6477998
doi:
Substances chimiques
DNA, Bacterial
0
DNA, Ribosomal
0
Fatty Acids
0
RNA, Ribosomal, 16S
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
101Références
Environ Microbiol. 2015 May;17(5):1615-30
pubmed: 25142831
Eur J Pediatr. 1996 Jul;155 Suppl 1:S6-10
pubmed: 8828600
ISME J. 2012 Aug;6(8):1535-43
pubmed: 22343308
Endocr Connect. 2016 Jan;5(1):1-9
pubmed: 26555712
Genome Biol. 2017 Apr 13;18(1):70
pubmed: 28407784
Gut. 1989 Jan;30(1):19-25
pubmed: 2920921
Nature. 2006 Dec 14;444(7121):860-7
pubmed: 17167474
Gut. 2019 Jun;68(6):1108-1114
pubmed: 30670574
Front Microbiol. 2017 Sep 22;8:1765
pubmed: 29018410
Eur J Nutr. 2019 Sep;58(6):2365-2376
pubmed: 30046942
Br J Nutr. 2010 Sep;104(5):693-700
pubmed: 20346190
Crit Rev Food Sci Nutr. 2019;59(19):3227-3236
pubmed: 30373382
Nutr Metab Cardiovasc Dis. 2017 Feb;27(2):176-182
pubmed: 28081989
Clin Biochem Rev. 2008 Feb;29(1):31-41
pubmed: 18566668
Acta Paediatr. 2004 Dec;93(12):1596-600
pubmed: 15841767
Curr Opin Microbiol. 2013 Jun;16(3):255-61
pubmed: 23831042
ISME J. 2017 Apr;11(4):841-852
pubmed: 28045459
Int J Obes (Lond). 2013 Feb;37(2):216-23
pubmed: 22410962
Pediatrics. 1963 Sep;32:338-43
pubmed: 14063511
ISME J. 2015 Jan;9(1):90-100
pubmed: 24936765
Mol Genet Metab. 2013;110 Suppl:S18-24
pubmed: 24055312
Appl Environ Microbiol. 2007 Aug;73(16):5261-7
pubmed: 17586664
Nature. 2014 Jan 23;505(7484):559-63
pubmed: 24336217
Nutr Metab Cardiovasc Dis. 2018 Apr;28(4):385-392
pubmed: 29502926
PLoS One. 2016 Jun 23;11(6):e0157513
pubmed: 27336782
Anaerobe. 2013 Feb;19:70-6
pubmed: 23168133
Am J Physiol Gastrointest Liver Physiol. 2015 Oct 1;309(7):G590-601
pubmed: 26251473
Genet Med. 2014 Feb;16(2):121-31
pubmed: 24385075
BMC Bioinformatics. 2012 Feb 14;13:31
pubmed: 22333067
Orphanet J Rare Dis. 2018 Jun 27;13(1):103
pubmed: 29945661
Science. 2011 Oct 7;334(6052):105-8
pubmed: 21885731
Front Pediatr. 2014 Jan 28;2:6
pubmed: 24479111
Gut Microbes. 2012 Jul-Aug;3(4):289-306
pubmed: 22572875
Proc Natl Acad Sci U S A. 2010 Aug 17;107(33):14691-6
pubmed: 20679230
Diabetol Metab Syndr. 2017 Aug 15;9:62
pubmed: 28814977
Gastroenterology. 2014 May;146(6):1525-33
pubmed: 24560870
Lancet Diabetes Endocrinol. 2017 Sep;5(9):743-756
pubmed: 28082082
PLoS One. 2016 Mar 01;11(3):e0149731
pubmed: 26930652
Gastroenterology. 2014 May;146(6):1564-72
pubmed: 24503132
Nature. 2011 May 12;473(7346):174-80
pubmed: 21508958
Nutr Metab (Lond). 2012 Feb 03;9(1):7
pubmed: 22305125
PLoS One. 2017 Jun 21;12(6):e0179739
pubmed: 28636668
Nat Methods. 2010 May;7(5):335-6
pubmed: 20383131
Ecology. 2009 Dec;90(12):3566-74
pubmed: 20120823