Fusaric acid-induced promoter methylation of DNA methyltransferases triggers DNA hypomethylation in human hepatocellular carcinoma (HepG2) cells.
Carcinoma, Hepatocellular
/ genetics
DNA (Cytosine-5-)-Methyltransferases
DNA Methylation
/ drug effects
DNA-Binding Proteins
/ genetics
Fusaric Acid
/ pharmacology
Gene Expression Regulation, Neoplastic
/ drug effects
Hep G2 Cells
Humans
Liver Neoplasms
/ genetics
MicroRNAs
/ genetics
Promoter Regions, Genetic
/ drug effects
Ubiquitination
/ drug effects
DNA methyltransferases
DNMT ubiquitination
Fusaric acid
MBD2
global DNA hypomethylation
miR-29b
promoter methylation
Journal
Epigenetics
ISSN: 1559-2308
Titre abrégé: Epigenetics
Pays: United States
ID NLM: 101265293
Informations de publication
Date de publication:
08 2019
08 2019
Historique:
pubmed:
8
5
2019
medline:
27
5
2020
entrez:
8
5
2019
Statut:
ppublish
Résumé
Fusaric acid (FA), a mycotoxin contaminant of maize, displays toxicity in plants and animals; however, its epigenetic mechanism is unknown. DNA methylation, an epigenetic modification that regulates gene expression, is mediated by DNA methyltransferases (DNMTs; DNMT1, DNMT3A, and DNMT3B) and demethylases (MBD2). The expression of DNMTs and demethylases are regulated by promoter methylation, microRNAs (miR-29b) and post-translational modifications (ubiquitination). Alterations in these DNA methylation modifying enzymes affect DNA methylation patterns and offer novel mechanisms of FA toxicity. We determined the effect of FA on global DNA methylation as well as a mechanism of FA-induced changes in DNA methylation by transcriptional (promoter methylation), post-transcriptional (miR-29b) and post-translational (ubiquitination) regulation of DNMTs and MBD2 in the human hepatocellular carcinoma (HepG2) cell line. FA induced global DNA hypomethylation (
Identifiants
pubmed: 31060424
doi: 10.1080/15592294.2019.1615358
pmc: PMC6615533
doi:
Substances chimiques
DNA-Binding Proteins
0
MBD2 protein, human
0
MIRN29a microRNA, human
0
MicroRNAs
0
DNA (Cytosine-5-)-Methyltransferases
EC 2.1.1.37
Fusaric Acid
JWJ963070N
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
804-817Références
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