Mesenchymal stem cells of Systemic Sclerosis patients, derived from different sources, show a profibrotic microRNA profiling.
Adipose Tissue
/ chemistry
Adult
Bone Marrow Cells
/ chemistry
Cell Differentiation
Cell Proliferation
Cells, Cultured
Female
Gene Expression Profiling
/ methods
Gene Expression Regulation
Gene Regulatory Networks
Humans
Mesenchymal Stem Cells
/ chemistry
MicroRNAs
/ genetics
Scleroderma, Systemic
/ genetics
Sequence Analysis, RNA
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
09 05 2019
09 05 2019
Historique:
received:
08
01
2019
accepted:
24
04
2019
entrez:
11
5
2019
pubmed:
11
5
2019
medline:
21
10
2020
Statut:
epublish
Résumé
Systemic Sclerosis (SSc) is a disease with limited therapeutic possibilities. Mesenchymal stem cells (MSCs)-therapy could be a promising therapeutic option, however the ideal MSCs source has not yet been found. To address this problem, we perform comparison between bone marrow (BM)-MSCs and adipose (A)-MSCs, by the miRs expression profile, to identify the gene modulation in these two MSCs source. MicroRNAs (miRs) are RNAs sequences, regulating gene expression and MSCs, derived from different tissues, may differently respond to the SSc microenvironment. The miRs array was used for the miRs profiling and by DIANA-mirPath tool we identified the biological functions of the dysregulated miRs. In SSc-BM-MSCs, 6 miRs were significantly down-regulated and 4 miRs up-regulated. In SSc-A-MSCs, 11 miRs were significantly down-regulated and 3 miRs up-regulated. Interestingly, in both the sources, the involved pathways included the senescence mechanisms and the pro-fibrotic behaviour. Furthermore, both the MSCs sources showed potential compensatory ability. A deeper knowledge of this miRs signature might give more information about some pathogenic steps of the disease and in the same time clarify the possible therapeutic role of autologous MSCs in the regenerative therapy in SSc.
Identifiants
pubmed: 31073190
doi: 10.1038/s41598-019-43638-0
pii: 10.1038/s41598-019-43638-0
pmc: PMC6509164
doi:
Substances chimiques
MicroRNAs
0
Types de publication
Comparative Study
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
7144Références
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