Regulation of bacterial surface attachment by a network of sensory transduction proteins.
Adhesins, Bacterial
/ genetics
Bacterial Adhesion
Caulobacter crescentus
/ genetics
Ecosystem
Environment
Gene Expression Regulation, Bacterial
Gene Regulatory Networks
Gene-Environment Interaction
Histidine Kinase
/ genetics
Polysaccharides, Bacterial
/ genetics
Promoter Regions, Genetic
Protein Binding
Signal Transduction
/ genetics
Transcription Factors
/ genetics
Transcription, Genetic
Journal
PLoS genetics
ISSN: 1553-7404
Titre abrégé: PLoS Genet
Pays: United States
ID NLM: 101239074
Informations de publication
Date de publication:
05 2019
05 2019
Historique:
received:
12
02
2019
accepted:
26
04
2019
revised:
22
05
2019
pubmed:
11
5
2019
medline:
4
12
2019
entrez:
11
5
2019
Statut:
epublish
Résumé
Bacteria are often attached to surfaces in natural ecosystems. A surface-associated lifestyle can have advantages, but shifts in the physiochemical state of the environment may result in conditions in which attachment has a negative fitness impact. Therefore, bacteria employ numerous mechanisms to control the transition from an unattached to a sessile state. The Caulobacter crescentus protein HfiA is a potent developmental inhibitor of the secreted polysaccharide adhesin known as the holdfast, which enables permanent attachment to surfaces. Multiple environmental cues influence expression of hfiA, but mechanisms of hfiA regulation remain largely undefined. Through a forward genetic selection, we have discovered a multi-gene network encoding a suite of two-component system (TCS) proteins and transcription factors that coordinately control hfiA transcription, holdfast development and surface adhesion. The hybrid HWE-family histidine kinase, SkaH, is central among these regulators and forms heteromeric complexes with the kinases, LovK and SpdS. The response regulator SpdR indirectly inhibits hfiA expression by activating two XRE-family transcription factors that directly bind the hfiA promoter to repress its transcription. This study provides evidence for a model in which a consortium of environmental sensors and transcriptional regulators integrate environmental cues at the hfiA promoter to control the attachment decision.
Identifiants
pubmed: 31075103
doi: 10.1371/journal.pgen.1008022
pii: PGENETICS-D-19-00242
pmc: PMC6530869
doi:
Substances chimiques
Adhesins, Bacterial
0
Polysaccharides, Bacterial
0
Transcription Factors
0
bacterial adhesins, polysaccharide
0
Histidine Kinase
EC 2.7.13.1
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
e1008022Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM087353
Pays : United States
Organisme : NIGMS NIH HHS
ID : R25 GM066522
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM007183
Pays : United States
Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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