A comprehensive analysis of the Lactuca sativa, L. transcriptome during different stages of the compatible interaction with Rhizoctonia solani.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
10 05 2019
10 05 2019
Historique:
received:
12
12
2018
accepted:
30
04
2019
entrez:
12
5
2019
pubmed:
12
5
2019
medline:
21
10
2020
Statut:
epublish
Résumé
The leafy green vegetable Lactuca sativa, L. is susceptible to the soil-born fungus Rhizoctonia solani AG1-IB. In a previous study, we reported on the transcriptional response of R. solani AG1-IB (isolate 7/3/14) during the interspecies interaction with L. sativa cv. Tizian by means of RNA sequencing. Here we present the L. sativa transcriptome and metabolome from the same experimental approach. Three distinct interaction zones were sampled and compared to a blank (non-inoculated) sample: symptomless zone 1, zone 2 showing light brown discoloration, and a dark brown zone 3 characterized by necrotic lesions. Throughout the interaction, we observed a massive reprogramming of the L. sativa transcriptome, with 9231 unique genes matching the threshold criteria for differential expression. The lettuce transcriptome of the light brown zone 2 presents the most dissimilar profile compared to the uninoculated zone 4, marking the main stage of interaction. Transcripts putatively encoding several essential proteins that are involved in maintaining jasmonic acid and auxin homeostasis were found to be negatively regulated. These and other indicator transcripts mark a potentially inadequate defence response, leading to a compatible interaction. KEGG pathway mapping and GC-MS metabolome data revealed large changes in amino acid, lignin and hemicellulose related pathways and related metabolites.
Identifiants
pubmed: 31076623
doi: 10.1038/s41598-019-43706-5
pii: 10.1038/s41598-019-43706-5
pmc: PMC6510776
doi:
Substances chimiques
Plant Proteins
0
Transcription Factors
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
7221Références
Virulence. 2014;5(7):722-32
pubmed: 25513773
Plant Cell. 2011 Jan;23(1):4-15
pubmed: 21278123
Fungal Biol. 2014 Sep-Oct;118(9-10):800-13
pubmed: 25209639
Theor Appl Genet. 2009 Feb;118(3):565-80
pubmed: 19005638
Annu Rev Phytopathol. 2005;43:205-27
pubmed: 16078883
Phytopathology. 2015 Sep;105(9):1220-8
pubmed: 25915441
Plant Cell. 1997 Aug;9(8):1279-87
pubmed: 9286106
Plant Dis. 2003 Jul;87(7):766-771
pubmed: 30812884
Nucleic Acids Res. 2015 Jul 1;43(W1):W566-70
pubmed: 25969447
Front Plant Sci. 2016 Jun 28;7:897
pubmed: 27446127
J Mol Biol. 1990 Oct 5;215(3):403-10
pubmed: 2231712
Proc Natl Acad Sci U S A. 2010 May 18;107(20):9452-7
pubmed: 20439716
J Exp Bot. 2003 Apr;54(385):1259-68
pubmed: 12654877
Plant Physiol. 2005 Nov;139(3):1291-303
pubmed: 16244149
Metabolomics. 2007 Sep;3(3):211-221
pubmed: 24039616
Science. 2007 Feb 23;315(5815):1098-103
pubmed: 17185563
New Phytol. 2018 Jan;217(2):771-783
pubmed: 29048113
Sci Rep. 2018 Feb 12;8(1):2817
pubmed: 29434322
Nucleic Acids Res. 2019 Jan 8;47(D1):D590-D595
pubmed: 30321428
Sci Rep. 2017 Jul 25;7(1):6383
pubmed: 28743967
BMC Bioinformatics. 2009 Feb 03;10:48
pubmed: 19192299
Front Plant Sci. 2017 Aug 17;8:1422
pubmed: 28861102
Annu Rev Plant Biol. 2009;60:379-406
pubmed: 19400727
J Biotechnol. 2018 Feb 10;267:12-18
pubmed: 29278726
Annu Rev Phytopathol. 2012;50:267-94
pubmed: 22726121
Plant Cell Environ. 2009 Sep;32(9):1211-29
pubmed: 19389052
J Plant Physiol. 2011 Jan 1;168(1):51-62
pubmed: 20674079
BMC Plant Biol. 2017 Apr 27;17(1):84
pubmed: 28449662
Phytopathology. 2017 Jun;107(6):740-748
pubmed: 28134594
Bioinformatics. 2016 Dec 15;32(24):3702-3708
pubmed: 27540267
Nucleic Acids Res. 2007 Jul;35(Web Server issue):W182-5
pubmed: 17526522
Sci Rep. 2017 Feb 06;7:41610
pubmed: 28165003
IEEE Trans Vis Comput Graph. 2014 Dec;20(12):1983-92
pubmed: 26356912
Bioinformatics. 2014 Aug 15;30(16):2247-54
pubmed: 24790157
Plant Physiol. 2011 Aug;156(4):2053-68
pubmed: 21653783
Nucleic Acids Res. 2017 Jan 4;45(D1):D353-D361
pubmed: 27899662
Mol Plant Microbe Interact. 2012 Aug;25(8):1083-92
pubmed: 22550958
J Exp Bot. 2016 Jun;67(13):3845-54
pubmed: 26994477
Plant Cell Environ. 2013 Nov;36(11):1992-2007
pubmed: 23534608
Arabidopsis Book. 2010;8:e0136
pubmed: 22303261
G3 (Bethesda). 2013 Apr 9;3(4):617-631
pubmed: 23550116
Mol Plant. 2015 Apr;8(4):595-611
pubmed: 25660409
Plant Physiol. 2009 Oct;151(2):936-48
pubmed: 19700558
PLoS One. 2015 Dec 21;10(12):e0144769
pubmed: 26690577
Mol Plant Microbe Interact. 2001 Oct;14(10):1261-4
pubmed: 11605966
J Biotechnol. 2015 Jun 10;203:19-21
pubmed: 25801332
Front Plant Sci. 2016 May 06;7:587
pubmed: 27200058
Arabidopsis Book. 2011;9:e0156
pubmed: 22303280
J Biotechnol. 2013 Aug 20;167(2):142-55
pubmed: 23280342
Nat Rev Genet. 2010 Aug;11(8):539-48
pubmed: 20585331
Plant Cell Environ. 2014 May;37(5):1250-8
pubmed: 24237261
Genome Biol. 2013 Apr 25;14(4):R36
pubmed: 23618408
Plant Physiol. 2002 Apr;128(4):1480-9
pubmed: 11950996
Ann Bot. 2013 Jun;111(6):1021-58
pubmed: 23558912
Trends Plant Sci. 2010 May;15(5):247-58
pubmed: 20304701
Mol Plant Microbe Interact. 2010 Jul;23(7):861-70
pubmed: 20521949
Plant J. 2009 Jun;58(6):927-39
pubmed: 19220788
Nucleic Acids Res. 2000 Jan 1;28(1):27-30
pubmed: 10592173
G3 (Bethesda). 2015 Oct 08;5(12):2655-69
pubmed: 26449254
PLoS One. 2017 May 9;12(5):e0177278
pubmed: 28486484
Trends Plant Sci. 2013 Jan;18(1):30-40
pubmed: 22974587
Annu Rev Plant Biol. 2005;56:165-85
pubmed: 15862093
Plant Cell Physiol. 2014 Sep;55(9):1660-8
pubmed: 25008976
Plant Cell. 1998 Nov;10(11):1817-32
pubmed: 9811791