Histone H1.5 binds over splice sites in chromatin and regulates alternative splicing.
Journal
Nucleic acids research
ISSN: 1362-4962
Titre abrégé: Nucleic Acids Res
Pays: England
ID NLM: 0411011
Informations de publication
Date de publication:
09 07 2019
09 07 2019
Historique:
accepted:
27
04
2019
revised:
17
04
2019
received:
01
05
2018
pubmed:
12
5
2019
medline:
26
2
2020
entrez:
12
5
2019
Statut:
ppublish
Résumé
Chromatin organization and epigenetic markers influence splicing, though the magnitudes of these effects and the mechanisms are largely unknown. Here, we demonstrate that linker histone H1.5 influences mRNA splicing. We observed that linker histone H1.5 binds DNA over splice sites of short exons in human lung fibroblasts (IMR90 cells). We found that association of H1.5 with these splice sites correlated with the level of inclusion of alternatively spliced exons. Exons marked by H1.5 had more RNA polymerase II (RNAP II) stalling near the 3' splice site than did exons not associated with H1.5. In cells depleted of H1.5, we showed that the inclusion of five exons evaluated decreased and that RNAP II levels over these exons were also reduced. Our findings indicate that H1.5 is involved in regulation of splice site selection and alternative splicing, a function not previously demonstrated for linker histones.
Identifiants
pubmed: 31076740
pii: 5488017
doi: 10.1093/nar/gkz338
pmc: PMC6614845
doi:
Substances chimiques
Chromatin
0
H1-5 protein, human
0
Histones
0
RNA Splice Sites
0
DNA
9007-49-2
RNA Polymerase II
EC 2.7.7.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
6145-6159Informations de copyright
© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.
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