Diverse presentations of cutaneous mosaicism occur in CYLD cutaneous syndrome and may result in parent-to-child transmission.


Journal

Journal of the American Academy of Dermatology
ISSN: 1097-6787
Titre abrégé: J Am Acad Dermatol
Pays: United States
ID NLM: 7907132

Informations de publication

Date de publication:
Dec 2019
Historique:
received: 22 02 2019
revised: 30 04 2019
accepted: 07 05 2019
pmc-release: 01 12 2019
pubmed: 16 5 2019
medline: 15 4 2020
entrez: 16 5 2019
Statut: ppublish

Résumé

Clusters of rare cylindroma or spiradenoma tumors are a recurrent clinical presentation, yet conventional genetic testing results in individuals with these tumors are frequently normal. To determine if genetic mosaicism accounts for such cases. A study of 6 cases from a series of 55 patients who met criteria for diagnostic gene testing for pathogenic CYLD variants over a 5-year period (2012-2017) was performed. A novel genetic assay was used to study DNA from peripheral blood leukocytes and, where possible, matched skin and tumor tissue. Two patients had mosaic pathogenic CYLD variants in both the blood and skin. One of these patients transmitted a pathogenic variant to her daughter, and we report the novel phenotype of a contiguous gene deletion syndrome involving CYLD. Two patients had recurrent pathogenic variants in skin tumors from a single cluster but none detectable in the blood. The remaining 2 patients had clinical features of mosaicism, but these cases were not solved with the assays used because of a lack of access of fresh tumor tissue. Genetic mosaicism should be considered in patients presenting with clustered cylindromas, because this may inform genetic testing and counseling of these patients.

Sections du résumé

BACKGROUND BACKGROUND
Clusters of rare cylindroma or spiradenoma tumors are a recurrent clinical presentation, yet conventional genetic testing results in individuals with these tumors are frequently normal.
OBJECTIVE OBJECTIVE
To determine if genetic mosaicism accounts for such cases.
METHODS METHODS
A study of 6 cases from a series of 55 patients who met criteria for diagnostic gene testing for pathogenic CYLD variants over a 5-year period (2012-2017) was performed. A novel genetic assay was used to study DNA from peripheral blood leukocytes and, where possible, matched skin and tumor tissue.
RESULTS RESULTS
Two patients had mosaic pathogenic CYLD variants in both the blood and skin. One of these patients transmitted a pathogenic variant to her daughter, and we report the novel phenotype of a contiguous gene deletion syndrome involving CYLD. Two patients had recurrent pathogenic variants in skin tumors from a single cluster but none detectable in the blood.
LIMITATIONS CONCLUSIONS
The remaining 2 patients had clinical features of mosaicism, but these cases were not solved with the assays used because of a lack of access of fresh tumor tissue.
CONCLUSION CONCLUSIONS
Genetic mosaicism should be considered in patients presenting with clustered cylindromas, because this may inform genetic testing and counseling of these patients.

Identifiants

pubmed: 31085270
pii: S0190-9622(19)30784-4
doi: 10.1016/j.jaad.2019.05.021
pmc: PMC6878220
pii:
doi:

Substances chimiques

CYLD protein, human EC 3.4.19.12
Deubiquitinating Enzyme CYLD EC 3.4.19.12

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

1300-1307

Subventions

Organisme : Wellcome Trust
Pays : United Kingdom

Informations de copyright

Copyright © 2019 American Academy of Dermatology, Inc. Published by Elsevier Inc. All rights reserved.

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Auteurs

Majid Arefi (M)

Institute of Genetic Medicine, Newcastle University, Newcastle upon Tyne, United Kingdom.

Valerie Wilson (V)

Clinical Genetics, Centre for Life, Newcastle upon Tyne, United Kingdom.

Siobhan Muthiah (S)

Institute of Genetic Medicine, Newcastle University, Newcastle upon Tyne, United Kingdom; Department of Dermatology, Royal Victoria Infirmary, Newcastle upon Tyne, United Kingdom.

Simon Zwolinski (S)

Clinical Genetics, Centre for Life, Newcastle upon Tyne, United Kingdom.

Dalvir Bajwa (D)

Institute of Genetic Medicine, Newcastle University, Newcastle upon Tyne, United Kingdom.

Paul Brennan (P)

Clinical Genetics, Centre for Life, Newcastle upon Tyne, United Kingdom.

Katie Blasdale (K)

Department of Dermatology, Royal Victoria Infirmary, Newcastle upon Tyne, United Kingdom.

David Bourn (D)

Clinical Genetics, Centre for Life, Newcastle upon Tyne, United Kingdom.

John Burn (J)

Institute of Genetic Medicine, Newcastle University, Newcastle upon Tyne, United Kingdom; Clinical Genetics, Centre for Life, Newcastle upon Tyne, United Kingdom.

Mauro Santibanez-Koref (M)

Institute of Genetic Medicine, Newcastle University, Newcastle upon Tyne, United Kingdom.

Neil Rajan (N)

Institute of Genetic Medicine, Newcastle University, Newcastle upon Tyne, United Kingdom; Department of Dermatology, Royal Victoria Infirmary, Newcastle upon Tyne, United Kingdom. Electronic address: neil.rajan@ncl.ac.uk.

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Classifications MeSH