Selective Kinase Inhibition Shows That Bur1 (Cdk9) Phosphorylates the Rpb1 Linker

Cyclin-Dependent Kinases / genetics Gene Deletion Histone Chaperones / metabolism Mutation Phosphorylation Protein Domains Protein Kinases / metabolism RNA Polymerase II / chemistry Saccharomyces cerevisiae / metabolism Saccharomyces cerevisiae Proteins / chemistry Serine / metabolism Transcriptional Elongation Factors / metabolism
Cdk9 P-TEFb RNA polymerase II Rpb1 Spt6

Journal

Molecular and cellular biology
ISSN: 1098-5549
Titre abrégé: Mol Cell Biol
Pays: United States
ID NLM: 8109087

Informations de publication

Date de publication:
01 08 2019
Historique:
received: 02 01 2019
accepted: 03 05 2019
pubmed: 16 5 2019
medline: 25 1 2020
entrez: 16 5 2019
Statut: epublish

Résumé

Cyclin-dependent kinases play multiple roles in RNA polymerase II transcription. Cdk7/Kin28, Cdk9/Bur1, and Cdk12/Ctk1 phosphorylate the polymerase and other factors to drive the dynamic exchange of initiation and elongation complex components over the transcription cycle. We engineered strains of the yeast

Identifiants

DOI: 10.1128/MCB.00602-18 PMID: 31085683 PMC: PMC6639251
pubmed: 31085683
pii: MCB.00602-18
doi: 10.1128/MCB.00602-18
pmc: PMC6639251
pii:
doi:

Substances chimiques

CTDK-I protein complex, S cerevisiae 0
Histone Chaperones 0
SPT6 protein, S cerevisiae 0
Saccharomyces cerevisiae Proteins 0
Transcriptional Elongation Factors 0
Serine 452VLY9402
Protein Kinases EC 2.7.-
Cyclin-Dependent Kinases EC 2.7.11.22
SGV1 protein, S cerevisiae EC 2.7.11.22
Kin28 protein kinase, S cerevisiae EC 2.7.11.23
RNA Polymerase II EC 2.7.7.-
RPB1 protein, S cerevisiae EC 2.7.7.-

Types de publication

Journal Article Research Support, N.I.H., Extramural

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : NIGMS NIH HHS
ID : R01 GM056663
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM116560
Pays : United States
Organisme : NIAID NIH HHS
ID : P50 AI150464
Pays : United States
Organisme : NIGMS NIH HHS
ID : P50 GM082545
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM064649
Pays : United States

Informations de copyright

Copyright © 2019 Chun et al.

Références

Mol Cell. 2009 Mar 27;33(6):752-62
pubmed: 19328068
Cell Chem Biol. 2018 Feb 15;25(2):135-142.e5
pubmed: 29276047
Mol Cell. 2012 Jan 27;45(2):158-70
pubmed: 22284676
Elife. 2017 Aug 16;6:
pubmed: 28826505
Mol Cell. 2009 Mar 27;33(6):738-51
pubmed: 19328067
Nature. 2018 Aug;560(7720):607-612
pubmed: 30135578
Nat Struct Mol Biol. 2010 Sep;17(9):1154-61
pubmed: 20802488
Mol Cell. 2015 Aug 20;59(4):576-87
pubmed: 26257281
Genes Dev. 2019 May 1;33(9-10):578-589
pubmed: 30846429
Proc Natl Acad Sci U S A. 2009 Apr 28;106(17):6956-61
pubmed: 19365074
Mol Cell Biol. 2002 Mar;22(5):1288-97
pubmed: 11839796
Nat Struct Biol. 2003 Sep;10(9):679-80
pubmed: 12942140
Chem Biol. 2007 Nov;14(11):1261-72
pubmed: 18022565
Nat Commun. 2018 Feb 7;9(1):543
pubmed: 29416031
J Biol Chem. 2015 Jan 16;290(3):1786-95
pubmed: 25429106
Mol Cell Biol. 2003 Oct;23(19):7005-18
pubmed: 12972617
Proc Natl Acad Sci U S A. 2007 Apr 3;104(14):5812-7
pubmed: 17392431
Mol Cell. 2016 Aug 4;63(3):433-44
pubmed: 27477907
J Biol Chem. 2004 Jun 11;279(24):24957-64
pubmed: 15047695
Methods Enzymol. 2006;409:329-45
pubmed: 16793410
Mol Cell Biol. 2009 Sep;29(17):4852-63
pubmed: 19581288
Mol Cell Biol. 2004 Feb;24(4):1721-35
pubmed: 14749387
Mol Cell. 2016 Jan 21;61(2):297-304
pubmed: 26799764
Chem Rev. 2013 Nov 13;113(11):8423-55
pubmed: 24040939
EMBO J. 2012 Aug 15;31(16):3494-505
pubmed: 22796944
Nature. 2018 Jun;558(7710):460-464
pubmed: 29899453
Nucleic Acids Res. 2015 Nov 16;43(20):9766-75
pubmed: 26275777
Nat Struct Mol Biol. 2015 May;22(5):396-403
pubmed: 25849141
Nat Struct Mol Biol. 2012 Nov;19(11):1108-15
pubmed: 23064645
Nat Chem Biol. 2018 Feb;14(2):163-170
pubmed: 29251720
EMBO J. 2007 Nov 14;26(22):4646-56
pubmed: 17948059
Nature. 2000 Sep 21;407(6802):395-401
pubmed: 11014197
Science. 2005 May 27;308(5726):1318-21
pubmed: 15919995
Cell Rep. 2017 Aug 1;20(5):1173-1186
pubmed: 28768201
Nature. 2014 Jul 31;511(7511):616-20
pubmed: 25043025
Chem Rev. 2013 Nov 13;113(11):8456-90
pubmed: 23952966
Mol Cell Biol. 2009 Oct;29(20):5455-64
pubmed: 19667075
Genetics. 2015 Nov;201(3):1031-45
pubmed: 26416482
J Biol Chem. 2007 May 11;282(19):14113-20
pubmed: 17376774
Elife. 2014 Apr 29;3:e02407
pubmed: 24843027
Elife. 2018 Nov 13;7:
pubmed: 30422115
Protein Expr Purif. 2005 May;41(1):207-34
pubmed: 15915565
Genes Dev. 2001 Dec 15;15(24):3319-29
pubmed: 11751637

Auteurs

Yujin Chun (Y)

Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts, USA.
Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, Utah, USA.

Yoo Jin Joo (YJ)

Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts, USA.
Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, Utah, USA.

Hyunsuk Suh (H)

Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts, USA.
Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, Utah, USA.

Gaëlle Batot (G)

Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts, USA.
Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, Utah, USA.

Christopher P Hill (CP)

Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts, USA.
Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, Utah, USA.

Tim Formosa (T)

Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts, USA.
Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, Utah, USA.

Stephen Buratowski (S)

Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts, USA SteveB@hms.harvard.edu.
Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, Utah, USA.

Articles similaires

FBXO22 inhibits colitis and colorectal carcinogenesis by regulating the degradation of the S2448-phosphorylated form of mTOR.

Minle Li, Xuan Chen, Pengfei Qu et al.
1.00
Animals TOR Serine-Threonine Kinases Colorectal Neoplasms Colitis Mice

Spatial multiplex analysis of lung cancer reveals that regulatory T cells attenuate KRAS-G12C inhibitor-induced immune responses.

Megan Cole, Panayiotis Anastasiou, Claudia Lee et al.
1.00
T-Lymphocytes, Regulatory Lung Neoplasms Proto-Oncogene Proteins p21(ras) Animals Humans

Pathogenic mitochondrial DNA mutations inhibit melanoma metastasis.

Spencer D Shelton, Sara House, Luiza Martins Nascentes Melo et al.
1.00
DNA, Mitochondrial Humans Melanoma Mutation Neoplasm Metastasis

Exploring structural diversity across the protein universe with The Encyclopedia of Domains.

Andy M Lau, Nicola Bordin, Shaun M Kandathil et al.
1.00
Databases, Protein Protein Domains Protein Folding Proteins Deep Learning

Classifications MeSH

questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines et peptides de signalisation intracellulaire › Protein-Serine-Threonine Kinases › Proline-directed protein kinases › Kinases cyclines-dépendantes › Selective Kinase Inhibition Shows That Bur1...
questionsmedicales.fr › Phénomènes génétiques › Mutagenèse › Délétion de séquence › Délétion de gène › Selective Kinase Inhibition Shows That Bur1...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Chaperons moléculaires › Chaperons d'histones › Selective Kinase Inhibition Shows That Bur1...
questionsmedicales.fr › Phénomènes génétiques › Variation génétique › Mutation › Selective Kinase Inhibition Shows That Bur1...
questionsmedicales.fr › Métabolisme › Phosphorylation › Selective Kinase Inhibition Shows That Bur1...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Conformation moléculaire › Conformation des protéines › Structure tertiaire des protéines › Domaines protéiques › Selective Kinase Inhibition Shows That Bur1...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Transferases › Phosphotransferases › Phosphotransferases (Alcohol Group Acceptor) › Protein kinases › Selective Kinase Inhibition Shows That Bur1...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Transferases › Phosphotransferases › Nucleotidyltransferases › RNA nucleotidyltransferases › DNA-directed RNA polymerases › RNA polymerase II › Selective Kinase Inhibition Shows That Bur1...
questionsmedicales.fr › Eucaryotes › Champignons › Levures › Saccharomyces › Saccharomyces cerevisiae › Selective Kinase Inhibition Shows That Bur1...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines fongiques › Protéines de Saccharomyces cerevisiae › Selective Kinase Inhibition Shows That Bur1...
questionsmedicales.fr › Acides aminés, peptides et protéines › Acides aminés › Acides aminés neutres › Sérine › Selective Kinase Inhibition Shows That Bur1...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Facteurs de transcription › Facteurs d'élongation transcriptionnelle › Selective Kinase Inhibition Shows That Bur1...