Neutrophil Extracellular Traps Profiles in Patients with Incident Systemic Lupus Erythematosus and Lupus Nephritis.
Adolescent
Adult
Autoantibodies
/ blood
Child
Comorbidity
DNA
/ immunology
Deoxyribonuclease I
/ blood
Endodeoxyribonucleases
/ blood
Enzyme-Linked Immunosorbent Assay
Extracellular Traps
/ immunology
Female
Humans
Incidence
Lupus Erythematosus, Systemic
/ blood
Lupus Nephritis
/ blood
Male
Middle Aged
Mutation
Neutrophils
/ immunology
Prospective Studies
Severity of Illness Index
Young Adult
DNASE ACTIVITY
DNASE LEVEL
DNASE MUTATIONS
NEUTROPHIL EXTRACELLULAR TRAPS
Journal
The Journal of rheumatology
ISSN: 0315-162X
Titre abrégé: J Rheumatol
Pays: Canada
ID NLM: 7501984
Informations de publication
Date de publication:
03 2020
03 2020
Historique:
accepted:
24
04
2019
pubmed:
17
5
2019
medline:
21
5
2021
entrez:
17
5
2019
Statut:
ppublish
Résumé
Neutrophil extracellular traps (NET) expose modified antigens for autoantibodies in vasculitis. Little is known about levels and removal pathways of NET in systemic lupus erythematosus (SLE), especially in lupus nephritis (LN). We determined circulating levels and defined NET removal in large subsets of patients with incident SLE (iSLE), some of whom had new-onset nephritis. Serum levels of NET (ELISA), DNase1/DNase1L3 (ELISA), and DNase activity (functional assay) were determined in 216 patients with iSLE [103 had incident LN (iLN)], in 50 patients with other primary glomerulonephritis, and in healthy controls. Serum NET levels were very high in iSLE/iLN compared to all groups of controls and correlated with anti-dsDNA, C3-C4, and proteinuria; iLN had the highest levels. DNase activity was decreased in iLN compared to SLE (20% had one-half DNase activity) despite similar serum levels of DNase1/DNase1L3. In these cases, pretreatment of serum with protein A restored DNase efficiency; 1 patient was homozygous for a c.289_290delAC variant of Patients with iLN have increased circulating NET and reduced DNase activity, the latter being explained by the presence of inhibitory substances in circulation and/or by rare
Identifiants
pubmed: 31092713
pii: jrheum.181232
doi: 10.3899/jrheum.181232
pmc: PMC6917988
mid: NIHMS1060176
doi:
Substances chimiques
Autoantibodies
0
DNA
9007-49-2
DNASE1L3 protein, human
EC 3.1.-
Endodeoxyribonucleases
EC 3.1.-
DNASE1 protein, human
EC 3.1.21.1
Deoxyribonuclease I
EC 3.1.21.1
Banques de données
ClinicalTrials.gov
['NCT02403115']
Types de publication
Journal Article
Multicenter Study
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
377-386Subventions
Organisme : NIAID NIH HHS
ID : R01 AI132949
Pays : United States
Références
Nat Rev Immunol. 2006 Mar;6(3):173-82
pubmed: 16498448
J Am Soc Nephrol. 2014 May;25(5):990-7
pubmed: 24385592
FEBS J. 2009 Feb;276(4):1059-73
pubmed: 19154352
Proc Natl Acad Sci U S A. 2010 May 25;107(21):9813-8
pubmed: 20439745
PLoS One. 2010 Aug 10;5(8):
pubmed: 20856893
Front Immunol. 2013 Jan 17;3:428
pubmed: 23335928
PLoS One. 2009 Dec 29;4(12):e8474
pubmed: 20041189
Nat Med. 2007 Apr;13(4):403-4
pubmed: 17415369
Nat Genet. 2001 Aug;28(4):313-4
pubmed: 11479590
Curr Rheumatol Rep. 2016 Dec;18(12):71
pubmed: 27812953
Immunity. 2006 Sep;25(3):417-28
pubmed: 16973389
Nat Genet. 2000 Jun;25(2):177-81
pubmed: 10835632
J Clin Immunol. 2017 Feb;37(2):123-132
pubmed: 27943079
J Exp Med. 2010 Aug 30;207(9):1853-62
pubmed: 20733033
Arthritis Rheum. 2013 Aug;65(8):2183-9
pubmed: 23666765
Autoimmun Rev. 2015 Aug;14(8):692-702
pubmed: 25888464
Autoimmun Rev. 2010 Mar;9(5):A277-87
pubmed: 20036343
Arthritis Rheum. 2012 Aug;64(8):2677-86
pubmed: 22553077
Science. 2004 Mar 5;303(5663):1532-5
pubmed: 15001782
Hum Mol Genet. 2004 Oct 15;13(20):2343-50
pubmed: 15333586
Am J Pathol. 2009 Jul;175(1):97-106
pubmed: 19528352
J Exp Med. 2005 Jul 18;202(2):321-31
pubmed: 16027240
J Am Soc Nephrol. 1999 Feb;10(2):413-24
pubmed: 10215343
Lupus. 2011 Jan;20(1):67-70
pubmed: 21233149
Front Immunol. 2012 Dec 14;3:380
pubmed: 23248629
Clin Chem. 1993 Apr;39(4):561-77
pubmed: 8472349
Clin Chim Acta. 2017 Jan;464:12-16
pubmed: 27826099
Front Immunol. 2012 Nov 29;3:360
pubmed: 23264775
Trends Immunol. 2009 Nov;30(11):513-21
pubmed: 19699684
J Clin Invest. 1985 Jul;76(1):206-12
pubmed: 3894419
J Immunol. 2012 Apr 1;188(7):3522-31
pubmed: 22345666
Clin Chem. 1993 Jun;39(6):1345-6
pubmed: 7684958
Cell. 2016 Jun 30;166(1):88-101
pubmed: 27293190
Science. 2004 Oct 8;306(5694):279-83
pubmed: 15345777
J Cell Biol. 2007 Jan 15;176(2):231-41
pubmed: 17210947