Effect of resveratrol on vitrified in vitro produced bovine embryos: Recovering the initial quality.
Animals
Blastocyst
/ drug effects
Cattle
Cryopreservation
/ methods
Cryoprotective Agents
/ pharmacology
Embryo Culture Techniques
/ methods
Embryonic Development
/ physiology
Female
Fertilization in Vitro
Glutathione
/ pharmacology
Oocytes
/ drug effects
Reactive Oxygen Species
/ metabolism
Resveratrol
/ pharmacology
Vitrification
/ drug effects
Antioxidant
Apoptosis
Blastocyst
Cryopreservation
Gene expression
Oxidative stress
Journal
Cryobiology
ISSN: 1090-2392
Titre abrégé: Cryobiology
Pays: Netherlands
ID NLM: 0006252
Informations de publication
Date de publication:
08 2019
08 2019
Historique:
received:
16
02
2019
revised:
14
05
2019
accepted:
15
05
2019
pubmed:
20
5
2019
medline:
24
3
2020
entrez:
20
5
2019
Statut:
ppublish
Résumé
Although vitrification is the current routine method for human embryo cryopreservation, it may cause detrimental effects. The aim of this study was to evaluate the effect of supplementing in vitro culture (IVC) media and/or vitrification solutions (VS) with Resveratrol on the presence of apoptotic markers, reactive oxygen species (ROS) level, glutathione (GSH) content and relative gene abundance. Abattoir-derived oocytes were matured and fertilized in vitro according to a standard procedure. Zygotes were cultured in IVC medium supplemented with or without 0.5 μM Resveratrol (C
Identifiants
pubmed: 31103673
pii: S0011-2240(19)30058-6
doi: 10.1016/j.cryobiol.2019.05.008
pii:
doi:
Substances chimiques
Cryoprotective Agents
0
Reactive Oxygen Species
0
Glutathione
GAN16C9B8O
Resveratrol
Q369O8926L
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
42-50Informations de copyright
Copyright © 2019 Elsevier Inc. All rights reserved.