Tracking carrier protein motions with Raman spectroscopy.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
20 05 2019
20 05 2019
Historique:
received:
29
01
2019
accepted:
25
04
2019
entrez:
22
5
2019
pubmed:
22
5
2019
medline:
7
6
2019
Statut:
epublish
Résumé
Engineering microbial biosynthetic pathways represents a compelling route to gain access to expanded chemical diversity. Carrier proteins (CPs) play a central role in biosynthesis, but the fast motions of CPs make their conformational dynamics difficult to capture using traditional spectroscopic approaches. Here we present a low-resource method to directly reveal carrier protein-substrate interactions. Chemoenzymatic loading of commercially available, alkyne-containing substrates onto CPs enables rapid visualization of the molecular cargo's local environment using Raman spectroscopy. This method could clarify the foundations of the chain sequestration mechanism, facilitate the rapid characterization of CPs, and enable visualization of the vectoral processing of natural products both in vitro and in vivo.
Identifiants
pubmed: 31110182
doi: 10.1038/s41467-019-10184-2
pii: 10.1038/s41467-019-10184-2
pmc: PMC6527581
doi:
Substances chimiques
Bacterial Proteins
0
Biological Products
0
Carrier Proteins
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
2227Subventions
Organisme : NIGMS NIH HHS
ID : R15 GM120704
Pays : United States
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