Post-manufacture loading of filaments and 3D printed PLA scaffolds with prednisolone and dexamethasone for tissue regeneration applications.
Animals
Anti-Inflammatory Agents
/ chemistry
Cell Proliferation
/ drug effects
Cells, Cultured
Dexamethasone
/ chemistry
Mice
Polyesters
/ chemistry
Polymers
/ chemistry
Prednisolone
/ chemistry
Printing, Three-Dimensional
Regeneration
/ drug effects
Surface Properties
/ drug effects
Tissue Engineering
/ methods
Tissue Scaffolds
3D printing
Controlled release
Dexamethasone
Fused deposition modeling
Poly(L-lactic acid)
Post-manufacture loading
Prednisolone
Regenerative medicine
Journal
European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V
ISSN: 1873-3441
Titre abrégé: Eur J Pharm Biopharm
Pays: Netherlands
ID NLM: 9109778
Informations de publication
Date de publication:
Aug 2019
Aug 2019
Historique:
received:
27
03
2019
revised:
17
05
2019
accepted:
17
05
2019
pubmed:
22
5
2019
medline:
26
11
2019
entrez:
22
5
2019
Statut:
ppublish
Résumé
Strategies to load prednisolone or dexamethasone in preformed poly(L-lactic acid) (PLA) filaments and 3D printed scaffolds were explored as a way of personalizing the drug, the dose and the release profile for regenerative medicine purposes. Instead of starting from a PLA filament preloaded with a given content of drug, we explored two more versatile strategies. The first one involved the soaking of PLA filaments into a drug solution prepared in a solvent that reversibly swelled PLA; during 3D printing the melting of PLA contributed to the efficient integration (encapsulation) of the drug inside the printed strand. The second strategy consisted in first printing the 3D PLA scaffolds followed by soaking in a suitable drug solution in order to exploit the higher specific surface of the printed strands compared to the filament. Sustained release profiles were recorded when either prednisolone or dexamethasone were loaded in preformed PLA filaments, while rapid release was recorded for 3D PLA scaffolds loaded after printing. The combination of the two proposed methods reported here opened the possibility of creating concentration gradients of different drugs in the same scaffold exhibiting distinct release patterns. Namely, the strand core contained an active ingredient to be slowly released, while the surface was covered with other active ingredient that could be rapidly delivered. The feasibility of this approach was confirmed through dual loading of dexamethasone in the filament and of prednisolone on the preformed scaffold. Drug-loaded scaffolds were characterized in terms of printability, structural characteristics (DSC, XRD), mechanical properties, biodegradation, and ability to promote cell attachment and proliferation. Finally, anti-inflammatory response and osteoinductive properties were verified in cell cultures.
Identifiants
pubmed: 31112767
pii: S0939-6411(19)30362-5
doi: 10.1016/j.ejpb.2019.05.018
pii:
doi:
Substances chimiques
Anti-Inflammatory Agents
0
Polyesters
0
Polymers
0
poly(lactide)
459TN2L5F5
Dexamethasone
7S5I7G3JQL
Prednisolone
9PHQ9Y1OLM
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
100-110Informations de copyright
Copyright © 2019 Elsevier B.V. All rights reserved.