Role of Ancillary Techniques in Biliary Cytopathology Specimens.
Bile Duct Neoplasms
/ diagnosis
Bile Ducts, Intrahepatic
/ metabolism
Biliary Tract
/ metabolism
Cholangiocarcinoma
/ diagnosis
Cytodiagnosis
/ methods
High-Throughput Nucleotide Sequencing
/ methods
Humans
Immunohistochemistry
/ methods
In Situ Hybridization, Fluorescence
/ methods
Sensitivity and Specificity
Ancillary testing
Bile duct
Brushings
Cytology
Pancreatic duct
Sensitivity
Journal
Acta cytologica
ISSN: 1938-2650
Titre abrégé: Acta Cytol
Pays: Switzerland
ID NLM: 0370307
Informations de publication
Date de publication:
2020
2020
Historique:
received:
08
10
2018
accepted:
14
02
2019
pubmed:
24
5
2019
medline:
25
1
2020
entrez:
24
5
2019
Statut:
ppublish
Résumé
Biliary brushing cytology has become the standard of practice for the investigation of strictures of the biliary and pancreatic duct systems. The methodology however has a limitation in that it has low diagnostic sensitivity when only cytologic evaluation is used. A number of testing methodologies have been applied to brushing specimens in an attempt to improve overall sensitivity without loss of specificity. These have included DNA ploidy analysis, immunocytochemistry, individual gene mutational analysis, fluorescence in-situ hybridization (FISH), and next generation sequencing (NGS). Currently, FISH coupled with routine cytology appears to be the method of choice for improving diagnostic sensitivity. NGS shows significant promise for improvement of diagnostic sensitivity.
Identifiants
pubmed: 31121596
pii: 000498976
doi: 10.1159/000498976
doi:
Types de publication
Journal Article
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
175-181Informations de copyright
© 2019 S. Karger AG, Basel.