Mitochondrial proteome profiling of Leishmania tropica.

Carrier Proteins Chaperonins Chromatography, Liquid Citric Acid Cycle Electron Transport Heat-Shock Proteins Leishmania / metabolism Leishmania tropica / metabolism Mitochondria / metabolism Mitochondrial Membranes / metabolism Mitochondrial Proteins / isolation & purification Protein Folding Proteome / metabolism Tandem Mass Spectrometry
LC-MS/MS Leishmania Mitochondria Proteome

Journal

Microbial pathogenesis
ISSN: 1096-1208
Titre abrégé: Microb Pathog
Pays: England
ID NLM: 8606191

Informations de publication

Date de publication:
Aug 2019
Historique:
received: 05 02 2019
revised: 17 04 2019
accepted: 14 05 2019
pubmed: 28 5 2019
medline: 14 8 2019
entrez: 25 5 2019
Statut: ppublish

Résumé

The mitochondrion of kinetoplastida has unique characteristics both in structure and function. To better understand the mitochondrial proteome of the Leishmania tropica promastigote stage, liquid chromatography coupled with mass spectrometry (LC/MS/MS) approach was used. In the wake of mitochondria isolation and purity validation, 1212 proteins were identified, among which approximately 44% of proteins belonged to the mitochondrial proteome. Several functions were enriched in mitochondrial proteome including tricarboxylic acid cycle and respiratory chain, protein folding, signalling, transport, lipid metabolism, amino acid, and nucleotide metabolism. Furthermore, the result of the present research was compared with the previous related studies. Gaining more information about vital metabolism of the cell and molecules can be used for therapeutic purposes.

Identifiants

DOI: 10.1016/j.micpath.2019.103542 PMID: 31125686
pubmed: 31125686
pii: S0882-4010(19)30218-9
doi: 10.1016/j.micpath.2019.103542
pii:
doi:

Substances chimiques

Carrier Proteins 0
Heat-Shock Proteins 0
Mitochondrial Proteins 0
Proteome 0
Chaperonins EC 3.6.1.-

Types de publication

Journal Article

Langues

eng

Pagination

103542

Informations de copyright

Copyright © 2019 Elsevier Ltd. All rights reserved.

Auteurs

Minoo Tasbihi (M)

Department of Medical Parasitology and Mycology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.

Faezeh Shekari (F)

Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran; Department of Biology, University of Science and Culture, Tehran, Iran. Electronic address: faezehshekari@royaninstitute.org.

Homa Hajjaran (H)

Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.

Leila Masoori (L)

Department of Medical Parasitology and Mycology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.

Ramtin Hadighi (R)

Department of Medical Parasitology and Mycology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran. Electronic address: rhadighi@gmail.com.

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Classifications MeSH

questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines de transport › Mitochondrial proteome profiling of Leishmania tropica.
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Chaperons moléculaires › Protéines du choc thermique › Chaperonines › Mitochondrial proteome profiling of Leishmania tropica.
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Chromatographie › Chromatographie en phase liquide › Mitochondrial proteome profiling of Leishmania tropica.
questionsmedicales.fr › Métabolisme › Voies et réseaux métaboliques › Cycle citrique › Mitochondrial proteome profiling of Leishmania tropica.
questionsmedicales.fr › Métabolisme › Voies et réseaux métaboliques › Transport d'électrons › Mitochondrial proteome profiling of Leishmania tropica.
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Chaperons moléculaires › Protéines du choc thermique › Mitochondrial proteome profiling of Leishmania tropica.
questionsmedicales.fr › Eucaryotes › Euglénozoaires › Kinetoplastida › Trypanosomatina › Leishmania › Mitochondrial proteome profiling of Leishmania tropica.
questionsmedicales.fr › Eucaryotes › Euglénozoaires › Kinetoplastida › Trypanosomatina › Leishmania › Leishmania tropica › Mitochondrial proteome profiling of Leishmania tropica.
questionsmedicales.fr › Cellules › Structures cellulaires › Fractions subcellulaires › Mitochondries › Mitochondrial proteome profiling of Leishmania tropica.
questionsmedicales.fr › Cellules › Structures cellulaires › Fractions subcellulaires › Membranes intracellulaires › Membranes mitochondriales › Mitochondrial proteome profiling of Leishmania tropica.
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines mitochondriales › Mitochondrial proteome profiling of Leishmania tropica.
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Pliage des protéines › Mitochondrial proteome profiling of Leishmania tropica.
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéome › Mitochondrial proteome profiling of Leishmania tropica.
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Spectrométrie de masse › Spectrométrie de masse en tandem › Mitochondrial proteome profiling of Leishmania tropica.