ZNA probe immobilized single-use electrodes for impedimetric detection of nucleic acid hybridization related to single nucleotide mutation.
Electrochemical impedance spectroscopy
Electrochemical nucleic acid biosensor
Factor V Leiden mutation
Multi-channel screen printed array of electrodes
ZNA
Zip nucleic acid
Journal
Analytica chimica acta
ISSN: 1873-4324
Titre abrégé: Anal Chim Acta
Pays: Netherlands
ID NLM: 0370534
Informations de publication
Date de publication:
13 Sep 2019
13 Sep 2019
Historique:
received:
16
12
2018
revised:
14
04
2019
accepted:
16
04
2019
entrez:
27
5
2019
pubmed:
28
5
2019
medline:
17
9
2019
Statut:
ppublish
Résumé
The development of a low-cost and disposable biosensing technologies has received a great interest of healthcare for the sensitive and reliable detection of single nucleotide mutation related to single nucleotide polymorphisms (SNPs). In the present study, an impedimetric biosensing platform based on zip nucleic acids (ZNA) was developed for the sensitive detection of Factor V Leiden (FV Leiden) mutation. After optimization of experimental parameters, the sequence selective hybridization between ZNA probe and target related to FV Leiden mutation was evaluated via electrochemical impedance spectroscopy technique (EIS) by measuring changes at the charge transfer resistance, R
Identifiants
pubmed: 31128758
pii: S0003-2670(19)30470-2
doi: 10.1016/j.aca.2019.04.036
pii:
doi:
Substances chimiques
DNA Probes
0
factor V Leiden
0
Carbon
7440-44-0
Factor V
9001-24-5
DNA
9007-49-2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
78-85Informations de copyright
Copyright © 2019 Elsevier B.V. All rights reserved.