Single Cell RNA Sequencing Identifies Subsets of Hepatic Stellate Cells and Myofibroblasts in Liver Fibrosis.
Actins
/ metabolism
Animals
Base Sequence
/ genetics
Carbon Tetrachloride
/ pharmacology
Cell Cycle Proteins
/ genetics
Cells, Cultured
Chemokines
/ genetics
Collagen
/ genetics
Disease Models, Animal
Gene Expression
Genetic Heterogeneity
Hepatic Stellate Cells
/ metabolism
Liver
/ cytology
Liver Cirrhosis
/ chemically induced
Mice
Mice, Inbred C57BL
Myofibroblasts
/ metabolism
Receptor, Platelet-Derived Growth Factor beta
/ metabolism
S100 Calcium Binding Protein A6
/ genetics
Sequence Analysis, RNA
hepatic stellate cells
liver fibrosis
myofibroblasts
scRNASeq
Journal
Cells
ISSN: 2073-4409
Titre abrégé: Cells
Pays: Switzerland
ID NLM: 101600052
Informations de publication
Date de publication:
24 05 2019
24 05 2019
Historique:
received:
01
05
2019
revised:
20
05
2019
accepted:
22
05
2019
entrez:
30
5
2019
pubmed:
30
5
2019
medline:
30
5
2019
Statut:
epublish
Résumé
Activation of hepatic stellate cells (HSCs) and their trans-differentiation towards collagen-secreting myofibroblasts (MFB) promote liver fibrosis progression. During chronic liver disease, resting HSCs become activated by inflammatory and injury signals. However, HSCs/MFB not only produce collagen, but also secrete cytokines, participate in metabolism, and have biomechanical properties. We herein aimed to characterize the heterogeneity of these liver mesenchymal cells by single cell RNA sequencing. In vivo resting HSCs or activated MFB were isolated from C57BL6/J mice challenged by carbon tetrachloride (CCl
Identifiants
pubmed: 31137713
pii: cells8050503
doi: 10.3390/cells8050503
pmc: PMC6562512
pii:
doi:
Substances chimiques
Actins
0
Cell Cycle Proteins
0
Chemokines
0
S100 Calcium Binding Protein A6
0
S100a6 protein, mouse
0
alpha-smooth muscle actin, mouse
0
Collagen
9007-34-5
Carbon Tetrachloride
CL2T97X0V0
Receptor, Platelet-Derived Growth Factor beta
EC 2.7.10.1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
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