Single cell expression analysis reveals anatomical and cell cycle-dependent transcriptional shifts during heart development.
Animals
Cell Cycle
Cluster Analysis
Computational Biology
Cytoskeleton
/ metabolism
Gene Expression Profiling
Gene Expression Regulation, Developmental
Genomics
Heart
/ embryology
Mice
Morphogenesis
Myocardium
/ metabolism
Myocytes, Cardiac
/ cytology
RNA
/ metabolism
Sarcomeres
/ metabolism
Sequence Analysis, RNA
Signal Transduction
Single-Cell Analysis
/ methods
Transcription, Genetic
Cell cycle
Compact myocardium
Embryonic heart
Ligand receptor interaction
Single cell RNA sequencing
Trabecular myocardium
Journal
Development (Cambridge, England)
ISSN: 1477-9129
Titre abrégé: Development
Pays: England
ID NLM: 8701744
Informations de publication
Date de publication:
14 06 2019
14 06 2019
Historique:
received:
12
11
2018
accepted:
15
05
2019
pubmed:
31
5
2019
medline:
24
6
2020
entrez:
31
5
2019
Statut:
epublish
Résumé
The heart is a complex organ composed of multiple cell and tissue types. Cardiac cells from different regions of the growing embryonic heart exhibit distinct patterns of gene expression, which are thought to contribute to heart development and morphogenesis. Single cell RNA sequencing allows genome-wide analysis of gene expression at the single cell level. Here, we have analyzed cardiac cells derived from early stage developing hearts by single cell RNA-seq and identified cell cycle gene expression as a major determinant of transcriptional variation. Within cell cycle stage-matched CMs from a given heart chamber, we found that CMs in the G2/M phase downregulated sarcomeric and cytoskeletal markers. We also identified cell location-specific signaling molecules that may influence the proliferation of other nearby cell types. Our data highlight how variations in cell cycle activity selectively promote cardiac chamber growth during development, reveal profound chamber-specific cell cycle-linked transcriptional shifts, and open the way to deeper understanding of pathogenesis of congenital heart disease.
Identifiants
pubmed: 31142541
pii: dev.173476
doi: 10.1242/dev.173476
pmc: PMC6602356
pii:
doi:
Substances chimiques
RNA
63231-63-0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NLM NIH HHS
ID : DP1 LM012179
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL145676
Pays : United States
Organisme : NHLBI NIH HHS
ID : R00 HL133472
Pays : United States
Organisme : NHLBI NIH HHS
ID : K99 HL133472
Pays : United States
Organisme : NHLBI NIH HHS
ID : T32 HL094274
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL141371
Pays : United States
Commentaires et corrections
Type : ErratumIn
Informations de copyright
© 2019. Published by The Company of Biologists Ltd.
Déclaration de conflit d'intérêts
Competing interestsThe authors declare no competing or financial interests.
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