WSL6 encoding an Era-type GTP-binding protein is essential for chloroplast development in rice.

Chloroplasts / physiology Cloning, Molecular GTP-Binding Proteins / metabolism Gene Expression Regulation, Plant Genetic Complementation Test Microscopy, Electron, Transmission Mutation Oryza / genetics Phenotype Pigmentation Plant Proteins / genetics Protein Biosynthesis RNA, Ribosomal, 16S / genetics Temperature
Era-type GTP-binding protein Oryza sativa PEP Ribosome biogenesis

Journal

Plant molecular biology
ISSN: 1573-5028
Titre abrégé: Plant Mol Biol
Pays: Netherlands
ID NLM: 9106343

Informations de publication

Date de publication:
Aug 2019
Historique:
received: 04 01 2019
accepted: 18 05 2019
pubmed: 31 5 2019
medline: 6 8 2019
entrez: 1 6 2019
Statut: ppublish

Résumé

Rice WSL6 is involved in chloroplast ribosome biogenesis and is essential for early chloroplast development. Construction of the genetic translation system is a prerequisite for chloroplast development in plants. However, the molecular mechanism underlying this process is largely unknown. Here, we isolated a white stripe leaf6 (wsl6) mutant in rice. The mutant seedlings displayed white-striped leaves that were more severe under low-temperature conditions. Transmission electron microscopy analysis showed that the wsl6 mutant was defective in early chloroplast development. Map-based cloning revealed that WSL6 encodes an Era-type guanosine-5'-triphosphate (GTP)-binding protein located in chloroplasts. Immunoblotting and quantitative real-time polymerase chain reaction (qRT-PCR) analyses demonstrated an absence of 70S ribosomes in wsl6 chloroplasts. Further research showed that WSL6 binds to the 16S ribosomal RNA (rRNA) subunit of chloroplast ribosome 30S. In summary, these results show that WSL6 is essential for chloroplast ribosome biogenesis during early chloroplast development in rice.

Identifiants

DOI: 10.1007/s11103-019-00885-z PMID: 31147815
pubmed: 31147815
doi: 10.1007/s11103-019-00885-z
pii: 10.1007/s11103-019-00885-z
doi:

Substances chimiques

Plant Proteins 0
RNA, Ribosomal, 16S 0
GTP-Binding Proteins EC 3.6.1.-

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

635-645

Subventions

Organisme : National Key R&D Program of China
ID : 2017YFD0100404
Organisme : National Key Transformation Program
ID : 2016ZX08001004

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Auteurs

Yinglun Sun (Y)

State Key Laboratory of Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing, 210095, China.

Yunlu Tian (Y)

State Key Laboratory of Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing, 210095, China.

Shuhan Cheng (S)

State Key Laboratory of Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing, 210095, China.

Yunlong Wang (Y)

State Key Laboratory of Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing, 210095, China.

Yuanyuan Hao (Y)

State Key Laboratory of Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing, 210095, China.

Jianping Zhu (J)

State Key Laboratory of Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing, 210095, China.

Xiaopin Zhu (X)

State Key Laboratory of Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing, 210095, China.

Yuanyan Zhang (Y)

State Key Laboratory of Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing, 210095, China.

Mingzhou Yu (M)

State Key Laboratory of Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing, 210095, China.

Jie Lei (J)

State Key Laboratory of Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing, 210095, China.

Xiuhao Bao (X)

State Key Laboratory of Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing, 210095, China.

Hongming Wu (H)

State Key Laboratory of Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing, 210095, China.

Yihua Wang (Y)

State Key Laboratory of Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing, 210095, China. yihuawang@njau.edu.cn.

Jianmin Wan (J)

State Key Laboratory of Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing, 210095, China. wanjm@njau.edu.cn.
National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agriculture Sciences, Beijing, 100081, China. wanjm@njau.edu.cn.

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Classifications MeSH

questionsmedicales.fr › Cellules › Structures cellulaires › Espace intracellulaire › Cytoplasme › Structures cytoplasmiques › Organites › Plastes › Chloroplastes › WSL6 encoding an Era-type GTP-binding protein...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Clonage moléculaire › WSL6 encoding an Era-type GTP-binding protein...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines et peptides de signalisation intracellulaire › Protéines G › WSL6 encoding an Era-type GTP-binding protein...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Régulation de l'expression des gènes végétaux › WSL6 encoding an Era-type GTP-binding protein...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Croisements génétiques › Test de complémentation › WSL6 encoding an Era-type GTP-binding protein...
questionsmedicales.fr › Techniques d'investigation › Microscopie électronique › Microscopie électronique à transmission › WSL6 encoding an Era-type GTP-binding protein...
questionsmedicales.fr › Phénomènes génétiques › Variation génétique › Mutation › WSL6 encoding an Era-type GTP-binding protein...
questionsmedicales.fr › Eucaryotes › Viridiplantae › Streptophyta › Embryophyta › Tracheobionta › Magnoliopsida › Poaceae › Oryza › WSL6 encoding an Era-type GTP-binding protein...
questionsmedicales.fr › Phénomènes génétiques › Phénotype › WSL6 encoding an Era-type GTP-binding protein...
questionsmedicales.fr › Phénomènes biologiques › Pigmentation › WSL6 encoding an Era-type GTP-binding protein...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines végétales › WSL6 encoding an Era-type GTP-binding protein...
questionsmedicales.fr › Phénomènes génétiques › Expression des gènes › Biosynthèse des protéines › WSL6 encoding an Era-type GTP-binding protein...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN ribosomique › ARN ribosomique 16S › WSL6 encoding an Era-type GTP-binding protein...
questionsmedicales.fr › Environnement et santé publique › Environnement › Concepts météorologiques › Atmosphère › Temps (météorologie) › Température › WSL6 encoding an Era-type GTP-binding protein...