Large-scale production of ursodeoxycholic acid from chenodeoxycholic acid by engineering 7α- and 7β-hydroxysteroid dehydrogenase.
Bile acid epimerization
Chenodeoxycholic acid
Hydroxysteroid dehydrogenase
Kilogram class
Ursodeoxycholic acid
Journal
Bioprocess and biosystems engineering
ISSN: 1615-7605
Titre abrégé: Bioprocess Biosyst Eng
Pays: Germany
ID NLM: 101088505
Informations de publication
Date de publication:
Sep 2019
Sep 2019
Historique:
received:
20
01
2019
accepted:
11
05
2019
pubmed:
4
6
2019
medline:
10
1
2020
entrez:
2
6
2019
Statut:
ppublish
Résumé
7α- and 7β-hydroxysteroid dehydrogenases (HSDHs) are key biocatalysts for the biotransformation of ursodeoxycholic acid (UDCA) from chenodeoxycholic acid (CDCA). Various researches focused on heterogeneously expressed engineering enzymes to epimerize CDCA for UDCA, however not yet applied to further industrial application. In this work, we present the large-scale production of UDCA from CDCA by 7α- and 7β-HSDH enzymatic synthesis. Engineering enzymes were both successfully heterologous overexpressed in Escherichia coli BL21, and the effect of the enzymatic synthesis was investigated. The mass analysis (MS), IR spectrum,
Identifiants
pubmed: 31152232
doi: 10.1007/s00449-019-02151-4
pii: 10.1007/s00449-019-02151-4
doi:
Substances chimiques
Chenodeoxycholic Acid
0GEI24LG0J
Hydroxysteroid Dehydrogenases
EC 1.1.-
7 beta-hydroxysteroid dehydrogenase
EC 1.1.1.-
7 alpha-hydroxysteroid dehydrogenase
EC 1.1.1.159
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM